Immunostimulatory nanocomplex

Abstract

The present invention relates to an immunostimulatory nanocomplex. The immunostimulatory nanocomplex comprises polyglutamic acid (PGA), a first positively charged substance, a second positively charged substance and a dengue viral protein for holding the dengue viral protein inside. The immunostimulatory nanocomplex is characterized by having a nonuniformally and positively charge distribution along a radial direction thereof. The nonuniformally and positively charge distribution comprises a first electrically charged portion having substantially electrical neutrality, a second electrically charged portion surrounding the first electrically charged portion, and a third electrically charged portion surrounding the second electrically charged portion. The third electrically charged portion has a third volume charge density more than a second volume charge density of the second electrically charged portion, thereby enhancing CD8(+) T-cell response and higher antibody titer after administrating an organism with the immunostimulatory nanocomplex.

What is claimed is: 
     
       1. An immunostimulatory nanocomplex comprising polyglutamic acid (PGA), a first positively charged substance, a second positively charged substance and a dengue viral protein, wherein the dengue viral protein is held inside the immunostimulatory nanocomplex, wherein the immunostimulatory nanocomplex has a nonuniform positive charge distribution along a radial direction thereof, and the nonuniform positive charge distribution comprises: a first electrically charged portion having a first volume charge density that is substantially neutral; a second electrically charged portion surrounding the first electrically charged portion; and a third electrically charged portion surrounding the second electrically charged portion, wherein the third electrically charged portion has a third volume charge density higher than a second volume charge density of the second electrically charged portion, and the third electrically charged portion comprises an outermost surface of the immunostimulatory nanocomplex. 
     
     
       2. The immunostimulatory nanocomplex according to  claim 1 , wherein the immunostimulatory nanocomplex has a zeta potential of 10 mV to 35 mV. 
     
     
       3. The immunostimulatory nanocomplex according to  claim 1 , wherein the first positively charged substance and the second positively charged substance are the same. 
     
     
       4. The immunostimulatory nanocomplex according to  claim 1 , wherein the first positively charged substance and the second positively charged substance are selected from the group consisting of chitosan (CS), gelatin, cationic cyclodextrin, cationic dextran, poly(L-lysine), polyethylenimine, and polyamidoamine. 
     
     
       5. The immunostimulatory nanocomplex according to  claim 1 , wherein the dengue viral protein comprises the sequence of SEQ ID NO 1 or 2.