Method to increase the yield of products in plant material

Abstract

A method or process to increase the production of products of interest in plant material including plant cultures, such as, for example, cell suspension cultures, root cultures, and hairy root cultures is provided. In one embodiment, the method is to contacting the plant material with a precursor or xenobiotic when producing a product of interest from a plant. In another embodiment the plant material is also contacted with a trapping agent. The process may also provide for contacting an elicitor of the product of interest with the plant material. An embodiment provides for contacting an elicitor, precursor and trapping agent with the plant material. The ability to produce novel compounds such as glucosides and glucuronides is provided.

What is claimed is: 
     
       1. A method of increasing the production of a phenolic from a hairy root culture through biosynthesis, the method comprising:
 contacting the hairy root culture capable of producing the phenolic with a cyclodextrin, a methyl jasmonate, and a magnesium chloride. 
 
     
     
       2. The method of  claim 1  further comprising:
 producing an increased amount of the phenolic compared to the method in which the hairy root culture is not contacted with the cyclodextrin, the methyl jasmonate, or the magnesium chloride. 
 
     
     
       3. The method of  claim 2  wherein the phenolic is a prenylated stilbenoid. 
     
     
       4. The method of  claim 2 , the hairy root culture further comprising:
 grape hairy roots. 
 
     
     
       5. The method of  claim 2  the hairy root culture further comprising:
 peanut hairy roots. 
 
     
     
       6. The method of  claim 3  wherein the prenylated stilbenoid comprises arachidin. 
     
     
       7. The method of  claim 1  wherein the methyl jasmonate, the cyclodextrin, and the magnesium chloride contact the hairy root culture for at least one hour. 
     
     
       8. The method of  claim 1  further comprising:
 contacting the hairy root culture with hydrogen peroxide. 
 
     
     
       9. The method of  claim 8  wherein the methyl jasmonate, the cyclodextrin, and the hydrogen peroxide contact the hairy root culture at the same time in an elicitation medium. 
     
     
       10. The method of  claim 6  wherein the methyl jasmonate, the cyclodextrin, and the magnesium chloride contact the hairy root culture at the same time in an elicitation medium. 
     
     
       11. A method of increasing the production of a prenylated stilbenoid from a hairy root culture through biosynthesis, the method comprising:
 contacting the hairy root culture capable of producing the prenylated stilbenoid with a cyclodextrin, a methyl jasmonate, and a magnesium chloride. 
 
     
     
       12. The method of  claim 11  wherein the prenylated stilbenoid comprises arachidin. 
     
     
       13. The method of  claim 11  wherein the methyl jasmonate, the cyclodextrin, and the magnesium chloride contact the hairy root culture for at least one hour. 
     
     
       14. The method of  claim 11  further comprising:
 contacting the hairy root culture with hydrogen peroxide. 
 
     
     
       15. The method of  claim 11  wherein the methyl jasmonate, the cyclodextrin, and the magnesium chloride contact the hairy root culture at the same time in an elicitation medium. 
     
     
       16. The method of  claim 14  wherein the methyl jasmonate, the cyclodextrin, and the hydrogen peroxide contact the hairy root culture at the same time in an elicitation medium. 
     
     
       17. A method of increasing the production of a phenolic from a hairy root culture through biosynthesis, the method comprising:
 contacting the hairy root culture capable of producing the phenolic with an elicitation medium comprising a cyclodextrin, a methyl jasmonate, and a magnesium chloride; and 
 producing an increased amount of the phenolic compared to the method in which the hairy root culture is not contacted with the cyclodextrin, the methyl jasmonate, or the magnesium chloride. 
 
     
     
       18. The method of  claim 17  wherein the phenolic is a prenylated stilbenoid. 
     
     
       19. The method of  claim 18 , the elicitation medium further comprising:
 hydrogen peroxide. 
 
     
     
       20. The method of  claim 19  wherein the elicitation medium contacts the hairy root culture for at least one hour.