Detergent compositions

Abstract

The present invention relates to detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning. The present invention furthermore relates to variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides.

The invention claimed is: 
     
       1. A detergent composition comprising a variant of a parent lipase which variant has lipase activity, has at least 90% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to D96E+T231R+N233R of SEQ ID NO: 2, wherein the amino acid at position 33 is asparagine (N);
 wherein said variant in comparison with the parent lipase has increased stability in the presence of detergents D001 or D002; and 
 wherein the variant has a half-life improvement factor above 2.0 as compared to an identical variant that has D at position 96. 
 
     
     
       2. The composition of  claim 1 , wherein the variant further comprises a substitution at position D27R of SEQ ID NO: 2. 
     
     
       3. The composition of  claim 1 , further comprising one or more enzymes selected from: hemicellulases, peroxidases, proteases, cellulases, xylanases, lipases, phospholipases, esterases, cutinases, pectinases, mannanases, pectate lyases, keratinases, reductases, oxidases, phenoloxidases, lipoxygenases, ligninases, pullulanases, tannases, pentosanases, malanases, ß-glucanases, arabinosidases, hyaluronidase, chondroitinase, laccase, chlorophyllases, amylases, or mixtures thereof. 
     
     
       4. The composition of  claim 1 , wherein the variant has at least 95% but less than 100%, sequence identity to SEQ ID NO: 2. 
     
     
       5. The composition of  claim 1 , further comprising at least one surfactant, at least one surfactant system, at least one soap, or any mixtures thereof. 
     
     
       6. The composition of  claim 1 , wherein the composition is a laundry cleaning composition, a dishwashing cleaning composition, a hard-surface cleaning composition or a personal care cleaning composition. 
     
     
       7. The composition of  claim 1 , wherein the composition is formulated as a regular, compact or concentrated liquid; a gel; a paste; a soap bar; a regular or a compacted powder; a granulated solid; a homogenous or a multilayer tablet with two or more layers (same or different phases); a pouch having one or more compartments; a single or a multi-compartment unit dose form; or any combination thereof. 
     
     
       8. A method of producing the composition according to  claim 1 , comprising adding a variant of a parent lipase which variant has lipase activity, has at least 90% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to D96E+T231R+N233R of SEQ ID NO: 2, wherein the amino acid at position 33 is asparagine (N),
 wherein said variant in comparison with the parent lipase has increased stability in the presence of detergents D001 or D002; and 
 wherein the half-life improvement factor of the variant is above 2.0 as compared to an identical variant having D at position 96. 
 
     
     
       9. A variant of a parent lipase, wherein the variant has 100% sequence identity with SEQ ID NO: 2, except that the variant has a group of substitutions selected from the group consisting of:
 a) D96E T231R N233R; 
 b) D27R G38A G91T D96E D111A G163K T231R N233R D254S P256T; 
 c) D96E T231R N233R D254S; 
 d) D27R G91T D96E D111A G163K T231R N233R D254S P256T; 
 e) D96E G163K T231R N233R D254S; 
 f) D27R G38A G91T D96E D111A G163K T231R N233R D254S; 
 g) D27R G38A G91T D96E G163K T231R N233R D254S P256T; 
 h) D27R G38A D96E D111A G163K T231R N233R D254S P256T; 
 i) D27R D96E G163K T231R N233R D254S; 
 j) D27R D96E D111A G163K T231R N233R D254S P256T; 
 k) D27R G38A D96E G163K T231R N233R D254S P256T; 
 l) D27R D96E D111A G163K T231R N233R; 
 m) D27R D96E D111AT231R N233R; 
 n) D27R G38A D96E D111A G163K E210Q T231R N233R D254S P256T; 
 o) D96E D111A G163K T231R N233R; 
 p) D96E D111A G163K T231R N233R D254S P256T; 
 q) D96E D111A G163K T231R N233R P256T; 
 r) D96E D111A T231R N233R; 
 s) D96E D111A T231R N233R D254S; 
 t) D96E D111A T231R N233R D254S P256T; 
 u) D96E D111A T231R N233R P256T; 
 v) D96E G163K T231R N233R D254S P256T; 
 w) D96E T231R N233R D254S P256T; 
 x) D96E T231R N233R P256T; 
 y) G38A D96E D111A T231R N233R; 
 z) G91T D96E D111A G163K T231R N233R D254S P256T; 
 aa) G91T D96E D111A T231R N233R; and 
 bb) G91T D96E T231R N233R; 
 
       wherein the variant has a half-life improvement factor above 1.5 as compared to an identical variant that has D at position 96. 
     
     
       10. The variant of  claim 9 , wherein said variant in comparison with the parent lipase has increased stability in the presence of detergents D001 or D002. 
     
     
       11. The variant of  claim 9 , wherein the half-life improvement factor of the variant is above 2.0 as compared to an identical variant having D at position 96. 
     
     
       12. A method for cleaning a subject, comprising contacting a lipid stain present on the subject to be cleaned with the composition according to  claim 1 .