Recombinant host cells for the production of malonate

Abstract

Compositions and methods for producing malonate in recombinant host cells in order to increase malonate titer, yield, and/or productivity are provided. Recombinant host cells comprising nucleic acids encoding MAE1 transport proteins that increase production of malonate by the host cell and vectors for expressing MAE1 transport proteins that increase production of malonate by the host cell are also disclosed.

The invention claimed is: 
     
       1. A recombinant  Pichia  kudriavzevii host cell capable of producing malonate wherein said host cell comprises a heterologous nucleic acid encoding an  Aspergillus  MAE1 transport protein, wherein the  Aspergillus  MAE1 transport protein is SEQ ID NO: 2, and wherein said host cell produces an increased level of malonate relative to a parental host cell not comprising a heterologous nucleic acid encoding said MAE1 transport protein. 
     
     
       2. A method for producing malonate, said method comprising fermenting a host cell of  claim 1  under conditions such that malonate is produced and secreted into fermentation medium. 
     
     
       3. The recombinant host cell of  claim 1 , which is capable of producing malonate under aerobic conditions. 
     
     
       4. The recombinant host cell of  claim 1 , which is capable of producing malonate under anaerobic conditions. 
     
     
       5. The recombinant host cell of  claim 1 , wherein the host cell produces greater than 1.25-fold more malonate than the parental host cell. 
     
     
       6. The method of  claim 2 , wherein the fermenting is performed at a pH of about 5.0. 
     
     
       7. The method of  claim 2 , wherein the fermenting is performed at a temperature of about 30° C.