Recombinant microorganism including genetic modification that increases pyruvate, phosphate dikinase activity and use thereof

Abstract

Provided are a recombinant microorganism including a genetic modification that increases a pyruvate, phosphate dikinase activity, a method of producing cellulose using the same, and a method of producing a microorganism having enhanced cellulose productivity.

What is claimed is: 
     
       1. A recombinant microorganism comprising
 a genetic modification that increases pyruvate, phosphate dikinase (PPDK) enzyme activity as compared with a microorganism without the genetic modification, wherein the PDDK enzyme belongs to EC 2.7.9.1, 
 and a genetic modification that decreases membrane-bound glucose dehydrogenase (GDH) activity as compared with a microorganism not comprising the genetic modification; 
 wherein the microorganism is  Clostridium, Komagataeibacter, Propionibacterium, Acetobacter, Agrobacterium , or  Escherichia.    
 
     
     
       2. The recombinant microorganism of  claim 1 , wherein the genetic modification that increases PDDK activity is a genetic modification that increases the copy number of a gene encoding the PPDK. 
     
     
       3. The recombinant microorganism of  claim 2 , wherein the PPDK gene is from  Clostridium, Komagataeibacter, Propionibacterium, Acetobacter, Agrobacterium , or  Escherichia.    
     
     
       4. The recombinant microorganism of  claim 1 , wherein PPDK is a polypeptide having 95% or higher sequence identity to any of SEQ ID NOs: 1 to 4. 
     
     
       5. The recombinant microorganism of  claim 2 , wherein the PPDK gene has 95% or higher sequence identity to any of SEQ ID NOs: 5 to 8. 
     
     
       6. The recombinant microorganism of  claim 1 , wherein the genetic modification that decreases membrane-bound glucose dehydrogenase activity is a genetic modification that inactivates or disrupts a gene encoding the membrane-bound glucose dehydrogenase. 
     
     
       7. A method of producing cellulose, the method comprising culturing a recombinant microorganism of  claim 1  in a medium; and separating cellulose from the culture. 
     
     
       8. The method of  claim 7 , wherein the genetic modification increases the copy number of a gene encoding the PPDK. 
     
     
       9. The method of  claim 7 , wherein the medium comprises 1 to 15 mM Mg′. 
     
     
       10. The method of  claim 7 , wherein the PPDK is a polypeptide having 95% or higher sequence identity to SEQ ID NO: 1. 
     
     
       11. The method of  claim 8 , wherein the PPDK gene has 95% or higher sequence identity to SEQ ID NO: 2. 
     
     
       12. The recombinant microorganism of  claim 1 , wherein the genetic modification that increases PDDK activity is a copy number increase in an exogenous gene encoding the PPDK. 
     
     
       13. The recombinant microorganism of  claim 1 , wherein the genetic modification that increases PDDK activity is a copy number increase in a heterologous gene encoding the PPDK. 
     
     
       14. The recombinant microorganism of  claim 1 , wherein the recombinant microorganism comprises a heterologous gene encoding PDDK, and a genetic modification that inactivates or disrupts a gene encoding the membrane-bound glucose dehydrogenase.