Bioactive aluminoborate glasses

Abstract

An aluminoborate glass composition, including B 2 O 3 , Al 2 O 3 , P 2 O 5 , Na 2 O, and CaO, as defined herein. Also disclosed are bioactive compositions including the disclosed aluminoborate glass composition, a suitable fluid, and at least one live cell. Also disclosed is method of limiting the amount of boron released into an aqueous solution from a disclosed aluminoborate-containing glass composition as defined herein. Also disclosed is a method of proliferating cells on a bioactive substrate as defined herein.

What is claimed is: 
     
       1. A bioactive composition, comprising:
 an aluminoborate glass composition, consisting of:
 30 to 60 mol % B 2 O 3 , 
 8 to 30 mol % Al 2 O 3 , 
 1 to 5 mol % P 2 O 5 , 
 3 to 30 mol % Na 2 O, 
 10 to 30 mol % CaO, and 
 balance of at least one alkali or alkaline earth metal oxide, based on a 100 mol % total of the composition; and 
 
 at least one live cell. 
 
     
     
       2. The bioactive composition of  claim 1 , wherein the at least one alkali or alkaline earth metal oxide comprises: 0.1 to 15 mol % K 2 O, 0.1 to 15 mol % MgO, or a combination thereof. 
     
     
       3. The bioactive composition of  claim 1  wherein the Al 2 O 3  content is from 10 to 15 mol %. 
     
     
       4. The bioactive composition of  claim 1 , wherein the at least one live cell is selected from the group consisting of an osteoblast, a keratinocyte, a human umbilical vein endothelial cell (HUVEC), or combinations thereof. 
     
     
       5. A method of controlling the rate of boron released into an aqueous solution from an aluminoborate-containing glass composition, comprising:
 contacting the aluminoborate-containing glass composition with an aqueous solution; wherein the aluminoborate-containing glass composition comprises sources of: 
 30 to 60 mol % B 2 O 3 , 
 8 to 30 mol % Al 2 O 3 , 
 1 to 5 mol % P 2 O 5 , 
 3 to 30 mol % Na 2 O, 
 10 to 30 mol % CaO, and 
 balance of at least one alkali or alkaline earth metal oxide, based on a 100 mol % total of the composition, 
 wherein the amount of Al 2 O 3  controls the amount of boron released into the aqueous solution or the boron release kinetics of the composition. 
 
     
     
       6. The method of  claim 5 , wherein the at least one alkali or alkaline earth metal oxide comprises 6 to 10 mol % K 2 O, 6 to 10 mol % MgO, or a combination thereof, in the aluminoborate-containing glass composition. 
     
     
       7. The method of  claim 5  further comprising measuring the boron concentration in the aqueous solution over time. 
     
     
       8. The method of  claim 5  wherein the amount of boron released into the aqueous solution from the aluminoborate-containing glass composition is reduced from 900 ppm to 50 ppm over a period of from 12 hrs to 10 days compared to an identical composition except that the identical composition is free of Al 2 O 3 . 
     
     
       9. The method of  claim 6  wherein the amount of boron released into the aqueous solution from the aluminoborate-containing glass composition is from 10 ppm to 100 ppm over a time period of from 0.5 to 10 days compared to an identical composition except that the identical composition is free of Al 2 O 3  and having a concentration of boron released of from 500 ppm to 1800 ppm over a time period of from 0.1 to 8 days. 
     
     
       10. The method of  claim 5  wherein the aqueous solution is a simulated body fluid (SBF) at a temperature of from 35 to 40° C. 
     
     
       11. A method of proliferating cells on a substrate comprising:
 contacting a substrate comprised of the aluminoborate glass composition of  claim 1  with a suitable liquid medium in the presence of at least one cell. 
 
     
     
       12. The method of  claim 11  wherein the contacting produces a proliferation of the at least one cell on the surface of the substrate by from 0.1 to 10 fold, compared to the proliferation in an alumina free composition. 
     
     
       13. The method of  claim 11  wherein the contacting produces a proliferation of the at least one cell in the suitable liquid medium, on the substrate, or both. 
     
     
       14. The method of  claim 11  wherein the suitable liquid medium includes a simulated body fluid composition and the at least one cell is a bone cell. 
     
     
       15. The method of  claim 14  wherein the contacting produces a hydroxyapatite (HA) compound on the surface of the substrate that stimulates in vitro or in vivo bonding to bone. 
     
     
       16. An article comprising:
 the aluminoborate glass composition of  claim 1 , wherein the article is at least one of biocompatible, angiogenic, or a combination thereof.