Microorganism with enhanced L-lysine producibility and method for producing L-lysine using the same

Abstract

The present disclosure relates to a novel modified RNA polymerase sigma factor A (SigA) polypeptide; a polynucleotide encoding the same; a microorganism containing the polypeptide; and a method for producing L-lysine using the microorganism.

The invention claimed is: 
     
       1. A modified polypeptide having RNA polymerase sigma factor A activity and the amino acid sequence set forth in SEQ ID NO: 2 but with one or two amino acid substitutions, wherein at least one of the one or two amino acid substitutions is selected from the group consisting of a substitution of the 136 th  amino acid with glycine; a substitution of the 254 th  amino acid with asparagine; a substitution of the 268 th  amino acid with serine; a substitution of the 281 st  amino acid with serine; a substitution of the 381 st  amino acid with arginine; a substitution of the 429 th  amino acid with arginine; a substitution of the 447 th  amino acid with histidine; a substitution of the 451 st  amino acid with isoleucine; a substitution of the 455 th  amino acid with valine; a substitution of the 479 th  amino acid with arginine; a substitution of the 483 rd  amino acid with arginine; a substitution of the 488 th  amino acid with threonine; and a substitution of the 491 st  amino acid with arginine. 
     
     
       2. The modified polypeptide according to  claim 1 , wherein the modified polypeptide has an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 12 to 22. 
     
     
       3. A polynucleotide encoding the modified polypeptide of  claim 1 . 
     
     
       4. A host cell comprising the polynucleotide of  claim 3 . 
     
     
       5. A microorganism of the genus  Corynebacterium  having enhanced L-lysine producibility, comprising a modified polypeptide having RNA polymerase sigma factor A activity and the amino acid sequence set forth in SEQ ID NO: 2 but with one or two substitutions in SEQ ID NO:2, wherein at least one of the one or two substitutions is selected from the group consisting of a substitution of the 136 th  amino acid with glycine; a substitution of the 254 th  amino acid with asparagine; a substitution of the 268 th  amino acid with serine; a substitution of the 281 st  amino acid with serine; a substitution of the 381 st  amino acid with arginine; a substitution of the 429 th  amino acid with arginine; a substitution of the 447 th  amino acid with histidine; a substitution of the 451 st  amino acid with isoleucine; a substitution of the 455 th  amino acid with valine; a substitution of the 479 th  amino acid with arginine; a substitution of the 483 rd  amino acid with arginine; a substitution of the 488 th  amino acid with threonine; and a substitution of the 491 st  amino acid with arginine. 
     
     
       6. The microorganism according to  claim 5 , wherein the microorganism is  Corynebacterium glutamicum.    
     
     
       7. A method for producing L-lysine, comprising:
 (a) culturing the microorganism according to  claim 5  in a medium; and 
 (b) recovering L-lysine from the cultured microorganism or the cultured medium.