Reagent calibration system and method

Abstract

One aspect relates to a method of calibrating event data. The method includes obtaining, via an electronic device including a processor, event data for an assay including a reagent. The reagent is associated with one of a plurality of manufacturing lots of the reagent. The method includes receiving one or more calibration factors for the reagent based on an identifier associated with the one of the plurality of manufacturing lots. The method further includes generating calibrated event data based on an application of the one or more calibration factors to the event data.

What is claimed is: 
     
       1. A method, comprising:
 obtaining, via an electronic device including a processor, event data for an assay, said assay associated with one of a plurality of manufacturing lots; 
 detecting, via the electronic device, an identifier affixed on packaging material for said assay, wherein the identifier is associated with the one of the plurality of manufacturing lots; 
 transmitting, via a network to a computing device different from the electronic device and the packaging material, a request for one or more calibration factors for said assay, wherein the request includes the identifier; 
 receiving, via the network, the one or more calibration factors for the one of the plurality of manufacturing lots of the assay; and 
 generating calibrated event data based on an application of the one or more calibration factors to the event data. 
 
     
     
       2. The method of  claim 1 , further comprising:
 calibrating a flow cytometer for the assay, wherein the flow cytometer is calibrated using at least one of the one or more calibration factors; and 
 analyzing, via the electronic device, a sample to generate the event data. 
 
     
     
       3. The method of  claim 1 , wherein receiving said one or more calibration factors includes:
 receiving a calibration factor for respective dimensions of spectral data detected using a reagent included in said assay. 
 
     
     
       4. The method of  claim 1 , further comprising:
 storing said one or more calibration factors in a machine-readable medium; and 
 retrieving said one or more calibration factors from the machine-readable medium. 
 
     
     
       5. The method of  claim 1 , wherein said one or more calibration factors include a mean fluorescence intensity calibration factor. 
     
     
       6. The method of  claim 1 , wherein the event data comprises flow cytometry event data. 
     
     
       7. The method of  claim 6 , wherein the flow cytometry event data includes median fluorescence intensity measurements. 
     
     
       8. The method of  claim 1 , wherein the request includes an instrument identifier for an instrument collecting the event data, and wherein the one or more calibration factors include a contextual adjustment for the instrument associated with the instrument identifier. 
     
     
       9. The method of  claim 8 , wherein the contextual adjustment is based on at least one of: a temperature of the instrument, memory available to the instrument, processor power available to the instrument, or an assay schedule of the instrument. 
     
     
       10. The method of  claim 1 , further comprising retrieving a calibration authorization value from a memory, wherein the request for the one or more calibration factors includes the calibration authorization value. 
     
     
       11. A system, comprising:
 an event data receiver configured to receive event data for an assay, said assay associated with one of a plurality of manufacturing lots; 
 a calibrator configured to:
 receive an identifier affixed on packaging material for said assay, wherein the identifier is associated with the one of the plurality of manufacturing lots; 
 transmit, via a network to a computing device different from the event data receiver and the packaging material, a request for one or more calibration factors for said assay, wherein there request includes the identifier; 
 receive, via the network, the one or more calibration factors for the one of the plurality of manufacturing lots of the assay; and 
 
 an event data processor configured to generate calibrated event data based on an application of the one or more calibration factors to the event data. 
 
     
     
       12. The system of  claim 11 , wherein the calibrator is further configured to calibrate a flow cytometer for the assay, wherein the flow cytometer is calibrated using at least one of the one or more calibration factors. 
     
     
       13. The system of  claim 11 , wherein the one or more calibration factors includes a calibration factor for respective dimensions of spectral data detected using a reagent included in said assay. 
     
     
       14. The system of  claim 11 , wherein said one or more calibration factors include a mean fluorescence intensity calibration factor. 
     
     
       15. The system of  claim 11 , wherein the event data comprises flow cytometry event data. 
     
     
       16. The system of  claim 15 , wherein the flow cytometry event data includes median fluorescence intensity measurements. 
     
     
       17. The system of  claim 11 , wherein the request includes an instrument identifier for an instrument collecting the event data, and wherein the one or more calibration factors include a contextual adjustment for the instrument associated with the instrument identifier. 
     
     
       18. The system of  claim 17 , wherein the contextual adjustment is based on at least one of: a temperature of the instrument, memory available to the instrument, processor power available to the instrument, or an assay schedule of the instrument. 
     
     
       19. The system of  claim 11 , wherein the packaging material includes a vessel containing a reagent used in said assay. 
     
     
       20. The system of  claim 11 , wherein the event data processor is further configured to associate the event data with the identifier associated with the one of the plurality of manufacturing lots.