Kit for quantitatively determining substance to be measured in biological sample and method for quantitatively determining substance to be measured in biological sample

Abstract

An object of the present invention is to provide a kit and a method by which a quantitative determination of a substance to be measured in a biological sample can be carried out with high accuracy while avoiding the influence of an antibody such as an anti-serum albumin antibody present in blood. According to the present invention, a kit for quantitatively determining a substance to be measured in a biological sample, the kit including: a labeled particle that has a first binding substance capable of binding to the substance to be measured, and a first blocking agent; and a substrate that has a detection region having a second binding substance capable of binding to any one of the substance to be measured and the first binding substance, and a second blocking agent, in which the first blocking agent and the second blocking agent are different from each other, is provided.

What is claimed is: 
     
       1. A kit for quantitatively determining a substance to be measured in a biological sample, the kit comprising:
 a labeled particle that has a first binding substance capable of binding to the substance to be measured, and a first blocking agent; and 
 a substrate that has a detection region having a second binding substance capable of binding to any one of the substance to be measured and the first binding substance, and a second blocking agent, 
 wherein the first blocking agent and the second blocking agent are different from each other, 
 the first blocking agent is one of albumins and an immunoglobulin other than an immunoglobulin capable of binding to the substances to be measured, and the second blocking agent is another one of albumins and an immunoglobulin other than an immunoglobulin capable of binding to the substances to be measured, and 
 the labeled particle is a latex particle and does not contain an emulsifier. 
 
     
     
       2. The kit according to  claim 1 ,
 wherein the albumin is a bovine serum albumin. 
 
     
     
       3. The kit according to  claim 1 ,
 wherein the labeled particle is a fluorescent particle, and 
 the detection region is on a gold film. 
 
     
     
       4. The kit according to  claim 2 ,
 wherein the labeled particle is a fluorescent particle, and 
 the detection region is on a gold film. 
 
     
     
       5. A method for quantitatively determining a substance to be measured in a biological sample, the method comprising:
 a reaction step of reacting a biological sample with a labeled particle that has a first binding substance capable of binding to the substance to be measured and has a first blocking agent; and 
 a substance to be measured-related label information acquisition step of bringing a reaction product obtained in the reaction step into contact with a substrate that has a detection region having a second binding substance capable of binding to any one of the substance to be measured and the first binding substance and having a second blocking agent, and capturing the labeled particle on the substrate so as to acquire label information related to an amount of the substance to be measured, 
 wherein the first blocking agent and the second blocking agent are different from each other, 
 the first blocking agent is one of albumins and an immunoglobulin other than an immunoglobulin capable of binding to the substances to be measured, and the second blocking agent is another one of albumins and an immunoglobulin other than an immunoglobulin capable of binding to the substances to be measured, and 
 the labeled particle is a latex particle and does not contain an emulsifier. 
 
     
     
       6. The method according to  claim 5 ,
 wherein the albumin is a bovine serum albumin. 
 
     
     
       7. The method according to  claim 5 ,
 wherein the labeled particle is a fluorescent particle, and 
 the detection region is on a gold film. 
 
     
     
       8. The method according to  claim 6 ,
 wherein the labeled particle is a fluorescent particle, and 
 the detection region is on a gold film. 
 
     
     
       9. The method according to  claim 7 ,
 wherein the label information related to an amount of the substance to be measured is acquired by detection of fluorescence resulting from surface plasmon excitation. 
 
     
     
       10. The method according to  claim 8 ,
 wherein the label information related to an amount of the substance to be measured is acquired by detection of fluorescence resulting from surface plasmon excitation.