Method and arrangement for acquiring image data

Abstract

A method and arrangement for acquiring image data, wherein a pixel area of a specimen to be imaged is illuminated in a plurality of illumination events with illumination light of a respective selected intensity, which intensities are different from one another. Subsequently, detection light emanating from the respective pixel area is acquired for each of the intensities as image data of a subpixel and resulting image data of the pixel area are determined from the number of acquired image data of the subpixels. According to the invention, the illumination events are triggered successively. The image data of all subpixels of the pixel area are acquired before a further pixel area is illuminated.

What is claimed is: 
     
       1. A method for acquiring image data, comprising
 illuminating a pixel area of a specimen to be imaged in a plurality of illumination events with illumination light of a respective selected intensity, which intensities are different from one another, 
 subsequently acquiring detection light emanating from the respective pixel area for each of the intensities as image data of a subpixel, and 
 determining resulting image data of the pixel area from the number of acquired image data of the subpixels, 
 wherein: said illumination events being triggered successively; and said image data of all subpixels of the pixel area being acquired before a further pixel area is illuminated, and 
 wherein: the intensities of successive illumination events of a pixel area are selected based on a step function, by virtue of function values of the step function being selected, control commands being generated based on the selected function values of the step function and an illumination device being actuated by way of the generated control commands. 
 
     
     
       2. The method as claimed in  claim 1 , wherein the successive illumination events are generated either with continuously increasing or with continuously decreasing intensities. 
     
     
       3. The method as claimed in  claim 1 , wherein the intensities of successive illumination events of a pixel area are selected based on a sinusoidal function, by virtue of function values of the sinusoidal function being selected, control commands being generated based on the selected function values of the sinusoidal function and an illumination device being actuated by way of the generated control commands. 
     
     
       4. A method for acquiring image data, comprising
 illuminating a pixel area of a specimen to be imaged in a plurality of illumination events with illumination light of a respective selected intensity, which intensities are different from one another, 
 subsequently acquiring detection light emanating from the respective pixel area for each of the intensities as image data of a subpixel, and 
 determining resulting image data of the pixel area from the number of acquired image data of the subpixels, 
 wherein: said illumination events being triggered successively; and said image data of all subpixels of the pixel area being acquired before a further pixel area is illuminated, and 
 generating the illumination events by use of a switching element, the temporal rise response of said switching element being able to be represented by a step function or a sinusoidal function. 
 
     
     
       5. The method as claimed in  claim 4 , wherein said switching element to generate the illumination events is an acousto-optic tunable filter.