Synthesis of long nucleic acid sequences

Abstract

The invention provides methods for the synthesis of long oligonucleotides, genes and gene fragments. The methods include the manufacture of genes or gene fragments that can be then inserted into a variety of vectors.

What is claimed is: 
     
       1. A gene fragment library, said library comprising: a plurality of non-vector synthetic nucleic acid gene fragments wherein two or more of the gene fragments are comprised of a constant gene block a  of at least 100 bases and a variable gene block of at least 50 bases, wherein the constant gene block sequence is identical for each of the gene fragments and the variable gene block sequence varies, and wherein the plurality of non-vector synthetic nucleic acid gene fragments are chemically synthesized. 
     
     
       2. The gene fragment library of  claim 1  wherein the gene fragments further comprise constant gene block b . 
     
     
       3. The gene fragment library of  claim 2  wherein the variable gene block is flanked by constant gene block a  and constant gene block b . 
     
     
       4. The gene fragment library of  claim 2  wherein the gene fragments further comprise constant gene block n  wherein n represents a plurality of sets of constant gene blocks. 
     
     
       5. The gene fragment library of  claim 1  wherein the gene fragments further comprise variable gene block n  wherein n represents a plurality of sets of variable gene blocks. 
     
     
       6. The gene fragment library of  claim 1  wherein the gene fragments are at least 400 bases. 
     
     
       7. The gene fragment library of  claim 1  wherein the gene fragments are at least 1000 bases. 
     
     
       8. The gene fragment library of  claim 3  wherein the constant gene blocks located on terminal ends contain a binding site for a forward primer on a first gene block located at a first terminal end and a reverse amplification primer on a second gene block located at a second terminal end. 
     
     
       9. The gene fragment library of  claim 1  wherein the constant gene blocks and the variable gene blocks contain overlap regions for assembly into a gene fragment. 
     
     
       10. The gene fragment library of  claim 9  wherein the overlap regions contain a same sequence of complementarity that allow for assembly with universal primers.