Method for monitoring cancer and/or inflammatory reaction based on RelB phosphorylation

Abstract

The present Inventors demonstrated that the RelB subunit of NFκB plays a crucial role in promoting cell migration. More precisely, they identified that this pro-migratory activity is mediated by the activation of the NFκB pathway through RelB phosphorylation at serine 472. In a first aspect, the present invention proposes to monitor the activation of the NFκB pathway by following the phosphorylation status of said serine. Also, the present invention discloses methods and kits for prognosing the evolution of a disease involving cell migration in a subject—treated or not—suffering thereof, based on the detection of said RelB-S472 phosphorylation.

The invention claimed is: 
     
       1. The monoclonal antibody produced by hybridoma RA3-AF3, deposited at the Collection Nationale de Cultures de Microorganismes (CNCM) at the Institut Pasteur, 25, Rue du Docteur Roux, 75724 Paris, FR, on Nov. 18, 2020, under the deposit number CNCM 1-5612. 
     
     
       2. A method, comprising:
 a) providing a sample; 
 b) contacting the sample with the monoclonal antibody of  claim 1 ; and 
 c) detecting binding between the monoclonal antibody and ReIB (SEQ ID NO: 1) phosphorylated on serine 472 or a ReIB-homologue having at least 80% identity to (SEQ ID NO: 1) and phosphorylated on a corresponding serine, in said sample. 
 
     
     
       3. The method of  claim 2 , wherein binding between the monoclonal antibody and ReIB (SEQ ID NO: 1) phosphorylated on serine 472 is detected.