Antibodies to ubiquitin C-terminal hydrolase L1 (UCH-L1) and glial fibrillary acidic protein (GFAP) and related methods

Abstract

The invention provides anti-UCH-L1 antibodies and anti-GFAP antibodies and their use in vitro detection of UCH-L1 and GFAP, respectively, in a sample from an individual, such as an individual known or suspected of having a brain injury or damage, for example, neurological damage such as mild traumatic brain injury. Also provided are methods, systems and kits for diagnosing brain injury or damage, such as neurological damage, in an individual with the aforementioned antibodies as well as compositions comprising the anti-UCH-L1 antibodies and anti-GFAP antibodies.

What is claimed is: 
     
       1. An antibody or antigen-binding fragment thereof that specifically binds to a human ubiquitin c-terminal hydrolase L I (UCH-L 1), wherein the antibody or antigen-binding fragment comprises a heavy chain variable region and a light chain variable region, wherein:
 the heavy chain variable region comprises: (i) a CDR-HL comprising the amino acid sequence of SEQ ID NO:1; (ii) a CDR-H2 comprising the amino acid sequence of SEQ ID NO:9; and (iii) a CDR-H3 comprising the amino acid sequence of SEQ ID NO:20; and 
 wherein the light chain variable region comprises: (i) a CDR-LI comprising the amino acid sequence of SEQ ID NO:28; (ii) a CDR-L2 comprising the amino acid sequence of SEQ ID NO:37; and (iii) a CDR-L3 comprising the amino acid sequence of SEQ ID NO:45. 
 
     
     
       2. A composition comprising the antibody or antigen-binding fragment of  claim 1 .