US11091771B2ActiveUtilityA1

PHI-4 polypeptides and methods for their use

71
Assignee: PIONEER HI BRED INTPriority: Mar 15, 2013Filed: May 8, 2019Granted: Aug 17, 2021
Est. expiryMar 15, 2033(~6.7 yrs left)· nominal 20-yr term from priority
Inventors:Ruth CongJingtong HouZhenglin HouPhillip A. PattenTakashi Yamamoto
C07K 14/245C07K 14/32A01N 37/46C07K 14/195A01N 43/50C07K 2319/02C12N 15/8286Y02A40/146C07K 14/24C07K 14/21
71
PatentIndex Score
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Cited by
15
References
24
Claims

Abstract

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.

Claims

exact text as granted — not AI-modified
That which is claimed: 
     
       1. A variant polypeptide selected from SEQ ID NO: 148; SEQ ID NO: 225; SEQ ID NO: 226; SEQ ID NO: 227; SEQ ID NO: 228; SEQ ID NO: 229; SEQ ID NO: 73; SEQ ID NO: 230; SEQ ID NO: 149; SEQ ID NO: 231; SEQ ID NO: 232; SEQ ID NO: 233; SEQ ID NO: 150; SEQ ID NO: 74; SEQ ID NO: 234; SEQ ID NO: 235; SEQ ID NO: 236; SEQ ID NO: 151; SEQ ID NO: 237; SEQ ID NO: 183; SEQ ID NO: 75; SEQ ID NO: 238; SEQ ID NO: 76; SEQ ID NO: 77; SEQ ID NO: 239; SEQ ID NO: 152; SEQ ID NO: 153; SEQ ID NO: 240; SEQ ID NO: 78; SEQ ID NO: 79; SEQ ID NO: 80; SEQ ID NO: 81; SEQ ID NO: 82; SEQ ID NO: 51; SEQ ID NO: 184; SEQ ID NO: 154; SEQ ID NO: 241; SEQ ID NO: 559; SEQ ID NO: 560; SEQ ID NO: 561; SEQ ID NO: 562; SEQ ID NO: 563; SEQ ID NO: 564; SEQ ID NO: 565; SEQ ID NO: 566; SEQ ID NO: 567; SEQ ID NO: 568; SEQ ID NO: 569; SEQ ID NO: 570; SEQ ID NO: 571; SEQ ID NO: 572; SEQ ID NO: 573; SEQ ID NO: 574; SEQ ID NO: 575; SEQ ID NO: 576; SEQ ID NO: 577; SEQ ID NO: 578; SEQ ID NO: 717; SEQ ID NO: 718; SEQ ID NO: 719; SEQ ID NO: 720; SEQ ID NO: 721; SEQ ID NO: 722; SEQ ID NO: 723; SEQ ID NO: 724; SEQ ID NO: 725; SEQ ID NO: 726; SEQ ID NO: 727; SEQ ID NO: 728; SEQ ID NO: 729; SEQ ID NO: 730; SEQ ID NO: 731; SEQ ID NO: 732; SEQ ID NO: 733; SEQ ID NO: 734; SEQ ID NO: 735; SEQ ID NO: 736; SEQ ID NO: 784; SEQ ID NO: 785; SEQ ID NO: 786; SEQ ID NO: 787; SEQ ID NO: 788; SEQ ID NO: 789; SEQ ID NO: 790; SEQ ID NO: 791; SEQ ID NO: 792; SEQ ID NO: 793; SEQ ID NO: 794; SEQ ID NO: 795; SEQ ID NO: 796; SEQ ID NO: 797; SEQ ID NO: 798; SEQ ID NO: 799; SEQ ID NO: 800; SEQ ID NO: 801; SEQ ID NO: 802; and SEQ ID NO: 803, wherein the variant polypeptide has at least 1.3 fold increased insecticidal activity against Western corn rootworm larvae compared to the polypeptide of SEQ ID NO: 35. 
     
     
       2. The variant polypeptide of  claim 1 , wherein the insecticidal activity is increased at least 2 fold or greater compared to the polypeptide of SEQ ID NO: 35. 
     
     
       3. The variant polypeptide of  claim 1 , wherein the insecticidal activity is increased at least 5 fold or greater compared to the polypeptide of SEQ ID NO: 35. 
     
     
       4. The variant polypeptide of  claim 1 , wherein the improved insecticidal activity compared to the polypeptide of SEQ ID NO: 35 is quantitated as a Mean FAE Index. 
     
     
       5. The variant polypeptide of  claim 1 , wherein the improved insecticidal activity compared to the polypeptide of SEQ ID NO: 35 is quantitated as an EC50 value. 
     
     
       6. The variant polypeptide of  claim 1 , wherein the improved activity compared to the polypeptide of SEQ ID NO: 35 is quantitated as a Mean Deviation Score. 
     
     
       7. A polynucleotide encoding the variant polypeptide of  claim 1 . 
     
     
       8. The polynucleotide of  claim 7 , wherein the insecticidal activity of the variant polypeptide is increased at least 2 fold or greater compared to the polypeptide of SEQ ID NO: 35. 
     
     
       9. The polynucleotide of  claim 7 , wherein the insecticidal activity of the variant polypeptide is increased at least 5 fold or greater compared to the polypeptide of SEQ ID NO: 35. 
     
     
       10. A composition, comprising an insecticidally-effective amount of the variant polypeptide of  claim 1 . 
     
     
       11. A method of inhibiting growth or killing of Western corn rootworm larvae, comprising contacting the Western corn rootworm larvae with the composition of  claim 10 . 
     
     
       12. A method for controlling a Western corn rootworm larvae population resistant to a pesticidal protein, comprising contacting the resistant Western corn rootworm larvae population with the composition of  claim 10 . 
     
     
       13. The method of  claim 12 , wherein the pesticidal protein is selected from Cry1Ac, Cry1Ab, Cry1A.105, Cry1Ac, Cry1F, Cry1Fa2, Cry1F, Cry2Ab, Cry3A, mCry3A, Cry3Bb1, Cry34Ab1, Cry35Ab1, Vip3A, Cry9c, eCry3.1Ab and CBI-Bt. 
     
     
       14. A transgenic plant or progeny thereof, comprising the polynucleotide of  claim 7 . 
     
     
       15. The transgenic plant or progeny thereof of  claim 14 , wherein the transgenic plant is a monocotyledon. 
     
     
       16. The transgenic plant or progeny thereof of  claim 15 , further comprising one or more additional transgenic traits. 
     
     
       17. Seed, grain or processed product thereof of the transgenic plant of  claim 14 , wherein the seed, grain, or processed product thereof comprises the polynucleotide of  claim 7 . 
     
     
       18. An expression cassette, comprising the polynucleotide of  claim 7  operably linked to one or more regulatory sequences directing expression of the variant polypeptide. 
     
     
       19. A transgenic plant or plant cell, comprising the expression cassette of  claim 18 . 
     
     
       20. A method for protecting a plant from Western corn rootworm larvae, comprising expressing in the plant or cell thereof, an insecticidally-effective amount of the variant polypeptide of  claim 1 . 
     
     
       21. A method for controlling a Western corn rootworm larvae population, comprising contacting the Western corn rootworm larvae population with an insecticidally-effective amount of the variant polypeptide of  claim 1 . 
     
     
       22. A method of inhibiting growth or killing of Western corn rootworm larvae, comprising contacting the Western corn rootworm larvae with a composition comprising an insecticidally-effective amount of the variant polypeptide of  claim 1 . 
     
     
       23. A method for controlling a Western corn rootworm larvae population resistant to a pesticidal protein, comprising contacting the Western corn rootworm larvae population with an insecticidally-effective amount of the variant polypeptide of  claim 1 . 
     
     
       24. A fusion protein comprising the variant polypeptide of  claim 1 .

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