Systems and methods for electrochemical triglycerides assays

Abstract

A system for the electrochemical detection of triglyceride levels includes a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area; and a coating on one of the electrode and counter electrode, the coating including a reagent coating for triglycerides.

The invention claimed is: 
     
       1. A system for the electrochemical detection of triglyceride levels, the system comprising:
 a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area; 
 a coating on one of the electrode and counter electrode, the coating including a reagent coating for triglycerides, the regent coating comprising a surfactant, a triglyceride hydrolyzing reagent, glycerol kinase, adenosine triphosphate, glycerol-3-phosphate dehydrogenase, a coenzyme, and a redox agent capable of being oxidized or reduced to form a product; and 
 an analyzer for receiving the test strip and including instructions stored on a non-transitory medium for applying a current or a voltage to the test strip and responsively determining an amount of triglycerides, wherein the redox agent is ferricyanide and an enzyme, wherein the enzyme is diaphorase, to catalyze a redox reaction. 
 
     
     
       2. The system of  claim 1 , wherein the triglyceride hydrolyzing reagent is lipoprotein lipase. 
     
     
       3. The system of  claim 1 , wherein the coenzyme is nicotinamide adenine dinucleotide (NAD). 
     
     
       4. The system of  claim 1 , wherein the surfactant includes a bile acid derivative. 
     
     
       5. The system of  claim 1 , wherein the surfactant includes a salt of a bile acid derivative. 
     
     
       6. A method of detecting triglycerides, the method comprising:
 providing an electrochemical test strip, the test strip including an electrode and a counter electrode, the electrode and counter electrode located in a sample reception area, and a coating on one of the electrode and counter electrode, the coating including a reagent coating for triglycerides, the reagent coating comprising a surfactant, a triglyceride hydrolyzing reagent, glycerol kinase, adenosine triphosphate, glycerol-3-phosphate dehydrogenase, a coenzyme, and a redox agent capable of being oxidized or reduced to form a product; 
 placing the electrochemical test strip in an analyzer; 
 placing a blood sample on the electrochemical test strip; 
 measuring a current provided through the blood sample and the electrochemical test strip; and 
 calculating a level of triglycerides with the analyzer based on the current, wherein the redox agent is ferricyanide and an enzyme, wherein the enzyme is diaphorase, to catalyze a redox reaction. 
 
     
     
       7. The method of  claim 6 , wherein the triglyceride hydrolyzing reagent is lipoprotein lipase. 
     
     
       8. The method of  claim 6 , wherein the coenzyme is nicotinamide adenine dinucleotide (NAD). 
     
     
       9. The method of  claim 6 , wherein the surfactant includes a bile acid derivative. 
     
     
       10. The method of  claim 6 , wherein the surfactant includes a salt of a bile acid derivative.