Biologic preserving composition and methods of use

Abstract

Disclosed herein are compositions, methods, and systems for treating skin damage or signs of aging in a subject including obtaining whole blood from the subject, collecting platelet rich plasma (PRP) from the whole blood, forming a first topical formulation having the PRP, providing the first topical formulation to the subject for application to an area of skin damage on the subject, providing a second topical formulation to the subject having an activator such that when the second topical formulation is applied to the area of skin damage on top of the first formulation, the PRP is activated and growth factors are released into the area of skin damage. Systems and kits useful in performing the methods are also disclosed.

What is claimed is: 
     
       1. A base composition for prolonging, preserving, or maintaining a biologic, the composition comprising in effective amounts therefor:
 a cell culture nutrient; 
 a biological buffer; 
 a viscosity modifying agent; 
 a botanical extract; and 
 one or more of benzalkonium chloride, benzoic acid or a salt or ester thereof, benzyl alcohol, a cresol, chloroxylenol, citric acid or a salt thereof, formic acid, O-cymen-5-ol, phenoxyethanol, a propionate salt, a salicylate salt, sorbate, triclosan, triclocarban, or glycinate, 
 wherein the composition is formulated as a topical formulation in the form of a cream, lotion, salve, paste, serum, gel, ointment, or foam. 
 
     
     
       2. The base composition of  claim 1 , wherein the cell culture nutrient comprises fetal bovine serum (FBS), glucose, human platelet lysate, bovine serum albumin, fibroblast growth supplement, vitamins, trace elements, antioxidants, minerals, amniotic cell culture supplements, or lipo-polysaccharides. 
     
     
       3. The base composition of  claim 1 , wherein the biological buffer comprises sodium, potassium, magnesium, calcium, alpha hydroxy acid, beta hydroxy acid, polyhydroxy acid, hyaluronic acid, carboxylic acid, or a cell culture buffering agent, or a derivative or any combination thereof, and wherein the biological buffer preserves the biologic at about or above physiological pH. 
     
     
       4. The base composition of  claim 1 , wherein the viscosity modifying agent comprises polyacrylate crosspolymer-6. 
     
     
       5. The base composition of  claim 1 , wherein the botanical extract comprises an aqueous ferment extract, an alcohol extract, a salicylate, a phenolic compound, or a phytonutrient. 
     
     
       6. The base composition of  claim 5 , wherein the aqueous ferment extract comprises a probiotic fermented botanical extract. 
     
     
       7. The base composition of  claim 6 , wherein the probiotic comprises  Lactobacillus, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus derivatives, Leuconostoc, Leuconostoc  derivatives,  Bifidobacterium, Bifidobacterium longum, Bifidobacterium derivatives, Streptococcus, Streptococcus thermophilus, Streptococcus derivatives, Saccharomyces  ferment filtrate, or  Bacillus  ferment. 
     
     
       8. The base composition of  claim 6 , wherein the probiotic fermented botanical extract comprises  Cocos nucifera  fruit fermented with  Lactobacillus, Leuconostoc  kimchi, or  Leuconostoc  with radish root ferment filtrate. 
     
     
       9. The base composition of  claim 5 , wherein the salicylate comprises an Aspen Bark isolate. 
     
     
       10. The base composition of  claim 5 , wherein the phenolic compound comprises a thymol. 
     
     
       11. The base composition of  claim 10 , wherein the thymol comprises a thymol isomer, a cresol, or O-cymen-5-ol. 
     
     
       12. The base composition of  claim 5 , wherein the phytonutrient is a  Sambucus nigra  fruit extract derivative,  Populus tremuloides  bark extract derivative, or  ribes nigrum  fruit extract derivative. 
     
     
       13. The base composition of  claim 1 , wherein the biologic is platelet rich plasma (PRP). 
     
     
       14. The base composition of  claim 1 , further comprising apple extract or apple skin extract. 
     
     
       15. The base composition of  claim 1 , further comprising ethylhexylglycerin, phenoxyethanol, EDTA, polysorbate, butylene glycol, xanthan gum, propanediol, tocopheryl acetate, glycerin, shea butter, water, one or more saccharides, or grape extract. 
     
     
       16. The base composition of  claim 1 , wherein the biological buffer is sodium citrate. 
     
     
       17. The base composition of  claim 1 , further comprising phenoxyethanol and capryl glycol.