Monoclonal antibody specifically binding to MRS

Abstract

The present invention relates to an anti-MRS monoclonal antibody and, more specifically, to an antibody or a fragment thereof characterized by specifically binding to a fragment represented by amino acid 861-900 of a human-derived methionyl-tRNA synthetase (MRS) protein set forth in SEQ ID NO:1, a method for producing the same, and a composition for diagnosing cancer comprising the same. The antibody or the fragment thereof of the present invention specifically binds to the human-derived MRS, and has no cross-reactivity with other proteins comprising the same ARS family. Therefore, as MRS detection is possible, the antibody or a fragment thereof can be effectively used for diagnosing MRS-related cancer.

What is claimed is: 
     
       1. An antibody or an antigen-binding fragment thereof which binds specifically to a peptide fragment consisting of the 861 st  to 900 th  amino acid residues of human-derived methionyl-tRNA synthetase (MRS) protein as set forth in SEQ ID NO:1,
 wherein the antibody or the fragment comprises:
 a light chain variable region (VL) comprising a light chain complementarity determining region 1 (CDR1) comprising an amino acid sequence as set forth in SEQ ID NO:3 or SEQ ID NO:15; a light chain complementarity determining region 2 (CDR2) comprising an amino acid sequence as set forth in SEQ ID NO:5 or SEQ ID NO:17; and a light chain complementarity determining region 3 (CDR3) comprising an amino acid sequence as set forth in SEQ ID NO:7 or SEQ ID NO:19; and 
 a heavy chain variable region (VH) comprising a heavy chain complementarity determining region 1 (CDR1) comprising an amino acid sequence as set forth in SEQ ID NO:9 or SEQ ID NO:21; a heavy chain complementarity determining region 2 (CDR2) comprising an amino acid sequence as set forth in SEQ ID NO:11 or SEQ ID NO:23; and a heavy chain complementarity determining region 3 (CDR3) comprising an amino acid sequence as set forth in SEQ ID NO:13 or SEQ ID NO:25. 
 
 
     
     
       2. The antibody or the fragment thereof of  claim 1 , wherein the antibody or the fragment comprises a heavy chain variable region and a light chain variable region selected from the group consisting of:
 the light chain variable region (VL) comprising the light chain complementarity determining region 1 (CDR1) comprising the amino acid sequence as set forth in SEQ ID NO:3, the light chain complementarity determining region 2 (CDR2) comprising the amino acid sequence as set forth in SEQ ID NO:5, and the light chain complementarity determining region 3 (CDR3) comprising the amino acid sequence as set forth in SEQ ID NO:7, and the heavy chain variable region (VH) comprising the heavy chain complementarity determining region 1 (CDR1) comprising an amino acid sequence as set forth in SEQ ID NO:9, the heavy chain complementarity determining region 2 (CDR2) comprising the amino acid sequence as set forth in SEQ ID NO:11, and the heavy chain complementarity determining region 3 (CDR3) comprising the amino acid sequence as set forth in SEQ ID NO:13; and 
 the light chain variable region (VL) comprising the light chain complementarity determining region 1 (CDR1) comprising the amino acid sequence as set forth in SEQ ID NO:15, the light chain complementarity determining region 2 (CDR2) comprising the amino acid sequence as set forth in SEQ ID NO:17, and the light chain complementarity determining region 3 (CDR3) comprising the amino acid sequence as set forth in SEQ ID NO:19, and the heavy chain variable region (VH) comprising the heavy chain complementarity determining region 1 (CDR1) comprising an amino acid sequence as set forth in SEQ ID NO:21, the heavy chain complementarity determining region 2 (CDR2) comprising the amino acid sequence as set forth in SEQ ID NO:23, and the heavy chain complementarity determining region 3 (CDR3) comprising the amino acid sequence as set forth in SEQ ID NO:25. 
 
     
     
       3. The antibody or the fragment thereof of  claim 1 , wherein the light chain variable region comprises a amino acid sequence as set forth in SEQ ID NO:27 or SEQ ID NO:31 and the heavy chain variable region comprises a amino acid sequence as set forth in SEQ ID NO:29 or SEQ ID NO:33. 
     
     
       4. The antibody or the fragment thereof of  claim 1 , wherein the antibody is selected from the group consisting of IgG, IgA, IgM, IgE and IgD, and the fragment thereof is selected from the group consisting of diabody, Fab, Fab′, F(ab)2, F(ab′)2, Fv and scFv.