Recombinant vector and method for producing recombinant fibroblast growth factor 19 using the same

Abstract

A recombinant vector according to an embodiment of the present invention may produce fibroblast growth factor 19 (FGF19) having enhanced solubility. The synonymous codon substitution variant fibroblast growth factor 19 (scvhFGF19) and chaperone ΔssDsbC may be simultaneously and independently expressed in a host cell into which the recombinant vector is introduced, thereby it is possible to overexpress the fibroblast growth factor 19 in a soluble state.

What is claimed is: 
     
       1. A recombinant vector for inducing soluble expression of fibroblast growth factor 19 (FGF19), the recombinant vector comprising:
 a first polynucleotide which encodes the fibroblast growth factor 19 (FGF19); and 
 a second polynucleotide which encodes disulfide bond isomerase (DsbC), 
 wherein the first polynucleotide consists of the sequence of SEQ ID NO: 3; 
 wherein the second polynucleotide does not comprise a signal sequence; 
 wherein the first polynucleotide and the second polynucleotide are operably linked to different promoters, respectively; and 
 wherein the recombinant vector is designed to introduce into  Escherichia coli.    
 
     
     
       2. The recombinant vector according to  claim 1 , wherein the second polynucleotide encodes a protein consisting of the amino acid sequence of SEQ ID NO: 2. 
     
     
       3. The recombinant vector according to  claim 1 ,
 wherein the second polynucleotide consists of the sequence of SEQ ID NO: 4. 
 
     
     
       4. A method for producing recombinant fibroblast growth factor 19 comprising culturing  Escherichia coli  transformed with the recombinant vector according to  claim 1 .