Genome-edited plant production method

Abstract

It was found that it is possible to construct a genome-edited plant in which no exogenous gene is incorporated in a genome by performing negative selection using morphological defects and the like as indices in a regenerated plant originated from a plant cell in which a gene that induces regeneration of the plant and a gene of the genome editing enzyme are introduced.

The invention claimed is: 
     
       1. A method for producing a genome-edited plant in which a mutation is introduced into a specificgene on a genome and no exogenousgene is incorporated on the genome, comprising the steps of:
 (a) introducing a construct that expresses a first gene encoding a genome editing enzyme targeting the specificgene on the genome and a second gene encoding a protein that induces or promotes regeneration of a plant into at least one plant cell, wherein said second gene is an isopentenyl transferase (ipt) gene; 
 (b) culturing the at least one plant cell obtained in the step (a); and 
 (c) selecting a regenerated plant exhibiting transient expression of said second gene, such that in said selected plant, said first and second genes are transiently expressed and are not incorporated into the genome, wherein the selected regenerated plant is the genome-edited plant. 
 
     
     
       2. The method according to  claim 1 , wherein
 the introduction of the construct into a plant cell in the step (a) is conducting by an agrobacterium method. 
 
     
     
       3. The method according to  claim 1 , wherein
 the culturing of the plant cell in the step (b) is conducted in a growth medium that contains no plant hormones. 
 
     
     
       4. The method according to  claim 1 , wherein
 a plant in which no morphological defect has occurred is selected.