Indirect ultrasonic cavitation-derived perivascular cells and methods of use thereof

Abstract

The present invention is directed to indirect ultrasonic cavitation-derived perivascular cells, to methods of use of a perivascular cell composition, to a method of processing a tissue and to an apparatus for the processing of a tissue. The methods include the mechanic indirect ultrasonication of a cellular non-structural tissue, and produce a perivascular fraction which includes perivascular cells. The methods of use are directed to the treatment of a variety of diseases and disorders and to the improvement of a tissue in a subject. The apparatus is provided for the processing of cellular non-structural tissue.

What is claimed is: 
     
       1. A method of isolating a perivascular cells (PVCs) from a cellular non-structural tissue comprising:
 a) indirectly sonicating the cellular non-structural tissue with an ultrasonic cavitation head in a closed and sterile system; and 
 b) collecting the PVCs, 
 
       wherein the ultrasonic cavitation head and cellular non-structural tissue are located in distinct containers, thereby isolating a PVCs without lysing or exploding PVCs. 
     
     
       2. The method of  claim 1 , wherein the PVCs comprise pericytes. 
     
     
       3. The method of  claim 1 , wherein the distance between the tissue and the ultrasonic cavitation head is about 0.1-20 millimeters. 
     
     
       4. The method of  claim 3 , wherein the distance between the tissue and the ultrasonic cavitation head is about 3-4 millimeters. 
     
     
       5. The method of  claim 1 , wherein the tissue is sonicated from about 1 minute to about 9 hours. 
     
     
       6. The method of  claim 5 , wherein the tissue is sonicated for about 5-15 minutes. 
     
     
       7. The method of  claim 1 , wherein the sonication is performed at about 15-50 kHz. 
     
     
       8. The method of  claim 1 , wherein the cellular non-structural tissue is human. 
     
     
       9. The method of  claim 1 , wherein the cellular non-structural tissue is obtained by surgical excision or aspiration. 
     
     
       10. The method of  claim 1 , further comprising differentiating the PVCs.