Hemostatic mixture of cellulose-based short and long fibers

Abstract

The invention relates to a hemostatic composition comprising a mixture of cellulose-based short and long fibers, preparation and use thereof.

The invention claimed is: 
     
       1. A method of treating a bleeding wound, a bacterial infection at a wound site, for sealing a leak at a site, preventing adhesion at a site, and/or minimizing or preventing a leak from an anastomotic site in a subject in need, the method comprising the step of applying onto and/or into the wound and/or site of the subject an effective amount of hemostatic fibers and/or aggregates composition comprising long and fine cellulose-based fibers, wherein the concentration of the long fibers is in the range of 5%-25% w/w and the concentration of the short fibers is in the range of 95%-75% w/w of the entire composition, wherein the size distribution of the long fibers is: D90 of more than 177 μm and D50 of more than 95 μm, and wherein the size distribution of the fine fibers is: D90 of less than 177 μm, and D50 of less than 95 μm. 
     
     
       2. The method according to  claim 1 , wherein the applying step is carried out without applying pressure on the composition towards the wound and/or site. 
     
     
       3. The method according to  claim 1 , wherein the D90 of the long fibers is less than 350 μm and the D50 is less than 167 μm. 
     
     
       4. The method according to  claim 1 , wherein the composition further comprises at least one compound selected from the group consisting of:
 a) a divalent cation selected from zinc, calcium, magnesium, manganese, and copper; 
 b) a positively charged peptide and/or polysaccharide; 
 c) an omega amino carboxylic acid; and 
 d) a combination of any of the above. 
 
     
     
       5. The method according to  claim 1 , wherein the composition further comprises protamine salt, calcium salt, and ε-aminocaproic acid (εACA). 
     
     
       6. The method according to  claim 1 , wherein the cellulose-based fibers comprise oxidized cellulose (OC) and/or oxidized regenerated cellulose (ORC) fibers. 
     
     
       7. The method according to  claim 1 , wherein the composition is in the form of aggregates having a size in the range of 75-420 μm.