Bivalent, bispecific binding proteins for prevention or treatment of HIV infection

Abstract

Provided herein are bivalent, bispecific binding proteins that specifically bind to two different HIV-1 Env protein epitopes.

What is claimed is: 
     
       1. An isolated nucleic acid molecule comprising a nucleotide sequence encoding a bispecific binding protein that specifically binds to two different HIV-1 Env protein epitopes, wherein the bispecific binding protein comprises a first and a second binding site;
 wherein the first binding site comprises V H1  and V L1 , wherein V H1  is a first immunoglobulin heavy chain variable domain that comprises the amino acid sequence of SEQ ID NO:506, and wherein V L1  is a first immunoglobulin light chain variable domain that comprises the amino acid sequence of SEQ ID NO:519; and 
 wherein the second binding site comprises V H2  and V L2 , wherein V H2  is a second immunoglobulin heavy chain variable domain that comprises the amino acid sequence of SEQ ID NO:504, and wherein V L2  is a second immunoglobulin light chain variable domain that comprises the amino acid sequence of SEQ ID NO:518. 
 
     
     
       2. An isolated nucleic acid molecule comprising a nucleotide sequence encoding a bispecific binding protein that specifically binds to two different HIV-1 Env protein epitopes, wherein the bispecific binding protein comprises a first and a second binding site;
 wherein the first binding site comprises V H1  and V L1 , wherein V H1  is a first immunoglobulin heavy chain variable domain that comprises the amino acid sequence of SEQ ID NO:503, and wherein V L1  is a first immunoglobulin light chain variable domain that comprises the amino acid sequence of SEQ ID NO:513; and 
 wherein the second binding site comprises V H2  and V L2 , wherein V H2  is a second immunoglobulin heavy chain variable domain that comprises the amino acid sequence of SEQ ID NO:506, and wherein V L2  is a second immunoglobulin light chain variable domain that comprises the amino acid sequence of SEQ ID NO:519. 
 
     
     
       3. An isolated nucleic acid molecule comprising a nucleotide sequence encoding a bispecific binding protein that specifically binds to two different HIV-1 Env protein epitopes, wherein the bispecific binding protein comprises a first and a second binding site;
 wherein the first binding site comprises V H1  and V L1 , wherein V H1  is a first immunoglobulin heavy chain variable domain that comprises the amino acid sequence of SEQ ID NO:506, and wherein V L1  is a first immunoglobulin light chain variable domain that comprises the amino acid sequence of SEQ ID NO:519; and 
 wherein the second binding site comprises V H2  and V L2 , wherein V H2  is a second immunoglobulin heavy chain variable domain that comprises the amino acid sequence of SEQ ID NO:503, and wherein V L2  is a second immunoglobulin light chain variable domain that comprises the amino acid sequence of SEQ ID NO:513.