Method of inhibiting oral pathogens

Abstract

A method of inhibiting oral pathogens comprising administering to a subject in need thereof an effective amount of Lactobacillus strain-containing food composite, medical composite and oral cleaning composite. The food composite, medical composite and oral cleaning composite comprises at least one Lactobacillus strain, which is at least one isolated Lactobacillus strain selected from a group including Lactobacillus acidophilus F-1 strain, CCTCC NO: M2011124; Lactobacillus salivarius subsp. salicinius AP-32 strain, CCTCC NO: M2011127; Lactobacillus reuteri GL-104 strain, CCTCC NO: M209138; Lactobacillus paracasei GL-156 strain, CCTCC NO: M2014590; Lactobacillus helveticus RE-78 strain, CGMCC No. 13513; Lactobacillus rhamnosus CT-53 strain, CCTCC NO: M2011129; and Lactobacillus paracasei ET-66 strain, CGMCC No. 13514, which are respectively preserved in China Center for Type Culture Collection (CCTCC) and China General Microbiological Culture Collection Center (CGMCC).

What is claimed is: 
     
       1. A method of inhibiting oral pathogens comprising administering to a human subject in need thereof a composite comprising:
 an isolated  Lactobacillus paracasei  ET-66 strain, CGMCC No. 13514; and 
 an excipient, diluent, or carrier, which is physiologically or medically acceptable, 
 wherein a number of the isolated  Lactobacillus paracasei  ET-66 strain, CGMCC No. 13514 is over 10 6  CFU. 
 
     
     
       2. The method according to  claim 1 , wherein the  Lactobacillus  strain is an active strain. 
     
     
       3. The method according to  claim 1 , wherein the composite further includes a  Lactobacillus  strain selected from the group consisting of  Lactobacillus acidophilus  F-1 strain, CCTCC NO: M2011124 ; Lactobacillus salivarius  subsp.  salicinius  AP-32 strain, CCTCC NO: M2011127 ; Lactobacillus reuteri  GL-104 strain, CCTCC NO: M209138 ; Lactobacillus rhamnosus  CT-53 strain, CCTCC NO: M2011129; and combinations thereof. 
     
     
       4. The method according to  claim 3 , wherein the  Lactobacillus  strain is a deactivated strain. 
     
     
       5. The method according to  claim 1 , wherein the isolated  Lactobacillus paracasei  ET-66 strain is a deactivated strain. 
     
     
       6. The method according to  claim 1 , wherein the oral pathogens include  Streptococcus mutans, Fusobacterium nucleatum  subsp.  polymorphum, Aggregatibacter actinomycetemcomitans , and  Porphyromonas gingivalis.    
     
     
       7. The method according to  claim 1 , wherein the composite is a food composite, medical composite, or oral cleaning composite. 
     
     
       8. The method according to  claim 7 , wherein the medical composite is in an oral form or a topical form. 
     
     
       9. The method according to  claim 1 , wherein the excipient or diluent is a food. 
     
     
       10. The method according to  claim 9 , wherein the food is selected from a milk-based drink, tea, coffee, a chewing gum, a tooth-cleaning candy, and combinations thereof. 
     
     
       11. The method according to  claim 1 , wherein the composite is an oral cleaning product selected from a toothpaste, a dental powder, a mouthwash, a fresh-breath spray, a fluorine-coating agent, and a false tooth cleaning agent. 
     
     
       12. The method according to  claim 1 , wherein the carrier is a toothbrush, an interdental brush, a dental floss, or an oral cotton stick. 
     
     
       13. The method according to  claim 1 , wherein the composite further includes another  Lactobacillus  strain selected from the group consisting of  Lactobacillus paracasei  GL-156 strain, CCTCC No: M2014590 ; Lactobacillus helveticus  RE-78 strain, CGMCC No. 13513; and a combination thereof. 
     
     
       14. The method according to  claim 13 , wherein the another  Lactobacillus  strain is a deactivated strain.