H5N6 recombinant influenza virus, a composition for preparing the same, and a vaccine composition containing the same

Abstract

The present invention relates to an mammalian non-pathogenic, highly-productive-in-embryonated-egg, heat-resistant, and attenuated clade 2.3.4.4c H5N6 recombinant influenza A virus, and a vaccine composition including the recombinant influenza virus and artificial H5 gene as active ingredients.

The invention claimed is: 
     
       1. An H5N6 recombinant influenza virus deposited as Accession No. KCTC14261BP, comprising one or more proteins selected from the group consisting of:
 a hemagglutinin (HA) protein of influenza virus H5N6 strain, wherein the hemagglutinin protein is a hemagglutinin protein, histidine (H), in which the 103 rd  amino acid from the N-terminus is mutated in the amino acid sequence of SEQ ID NO: 1 to tyrosine (Y); 
 neuraminidase (NA) of H5N6 strain; 
 polymerase subunit B2 (PB2) of low pathogenic influenza virus; 
 polymerase subunit B1 (PB1) of influenza virus H1N1 strain; 
 polymerase subunit A (PA) of influenza virus H1N1 strain; 
 nucleocapsid (nucleoprotein: NP) of influenza virus H1N1 strain or influenza virus H5N1 strain; 
 matrix protein (matrix: M) of influenza virus H1N1 strain or low pathogenic influenza virus; and 
 nonstructural protein (NS) of influenza virus H1N1 strain or influenza virus H9N2. 
 
     
     
       2. The H5N6 recombinant influenza virus of  claim 1 , wherein the low pathogenic influenza virus is low pathogenic influenza virus 01310 strain. 
     
     
       3. The H5N6 recombinant influenza virus of  claim 1 , wherein the influenza virus H1N1 strain is A/Puerto Rico/8/34(PR8). 
     
     
       4. The H5N6 recombinant influenza virus of  claim 1 , wherein the influenza virus H5N1 strain is A/wild duck/Korea/SNU50-5/2009. 
     
     
       5. The H5N6 recombinant influenza virus of  claim 1 , wherein the influenza virus H9N2 strain is A/chicken/Korea/KBNP-0028/00. 
     
     
       6. A vaccine composition, comprising the H5N6 recombinant influenza virus of  claim 1 .