Analyte depletion for sensor equilibration

Abstract

Methods include treating a portion of a sample composition to be tested for presence of an analyte by depleting or blocking the target analyte. The treated composition may be used to equilibrate an acoustic wave sensor prior to exposing the sensor to the untreated sample composition for analysis. By using the treated sample composition, in which the analyte is depleted or blocked, to equilibrate the sensor, the sensor may be equilibrated with a composition having a similar viscosity and non-specific binding characteristics to the untreated sample composition, which should result in improved accuracy when analyzing the analyte in the untreated sample composition.

What is claimed is: 
     
       1. A method for evaluating analyte presence comprising:
 providing a composition to be tested for the presence of an analyte; 
 contacting a first portion of the composition with a material configured to remove or block the analyte from the composition; and 
 flowing the first portion of the composition over a surface of a sensor, wherein an analyte capture ligand is bound to the surface of the sensor. 
 
     
     
       2. The method of  claim 1 , wherein the sensor is an acoustic wave sensor, configured to provide an output wave propagation characteristic to be monitored while the first portion of the composition is flowing over the surface of the acoustic wave sensor. 
     
     
       3. The method of  claim 2 , further comprising flowing a second portion of the composition over the surface of the sensor, wherein the second portion of the composition has not been contacted with the material configured to remove or block the analyte. 
     
     
       4. The method of  claim 3 , further comprising correlating a change in a monitored characteristic when the second portion of the composition is flowed over the surface of the sensor relative to a monitored characteristic when the first portion of the composition is flowed over the surface of the sensor to concentration of analyte in the second portion of the composition. 
     
     
       5. The method of  claim 1 , wherein the material configured to remove or block the analyte from the composition comprises the analyte capture ligand. 
     
     
       6. The method of  claim 1 , wherein contacting the first portion of the composition with the material configured to remove or block the analyte from the composition comprises flowing the first portion of the composition over or through the material. 
     
     
       7. The method of  claim 6 , wherein flowing the first portion of the composition over or through the material comprises flowing the first portion of the composition through a channel, wherein the material is disposed on a surface of the channel or disposed in the channel. 
     
     
       8. The method of  claim 1 , further comprising placing the first portion of the composition in a chamber. 
     
     
       9. The method of  claim 8 , wherein the chamber contains the material configured to remove or block the analyte from the composition when the first portion of the composition is placed in the chamber. 
     
     
       10. The method of  claim 9 , wherein the placing of the first portion of the composition in the chamber occurs when the composition is introduced into a port of a device configured to determine the presence of an analyte in the composition. 
     
     
       11. The method of  claim 1 , further comprising flowing the first portion of the composition over the surface of the sensor after the first portion has contacted the material configured to remove or block the analyte from the composition. 
     
     
       12. A device for evaluating analyte presence, comprising:
 a sensor having analyte capture ligand bound thereto; 
 a first fluid flow path positioned upstream of the sensor, configured to transport a first amount of a composition to be tested for analyte presence to the sensor; 
 a material configured to remove or block an analyte from the composition; 
 wherein the material configured to remove or block the analyte from the composition is positioned in the first fluid flow path. 
 
     
     
       13. The device of  claim 12 , further comprising:
 a second fluid flow path, positioned upstream of the sensor, and configured to transport a second amount of the composition to be tested for analyte presence to the sensor; 
 wherein the material configured to remove or block the analyte from the composition is not positioned in the second fluid flow path. 
 
     
     
       14. The device of  claim 13 , further comprising a first port configured to permit composition from the first fluid flow path to flow to the sensor, and a second port configured to permit composition from the second fluid flow path to flow to the sensor. 
     
     
       15. The device of  claim 12 , wherein the sensor is an acoustic wave sensor. 
     
     
       16. A method for equilibration of a sensor for testing the presence of an analyte, comprising:
 providing a composition to be tested for the presence of the analyte; 
 contacting a first portion of the composition with a material configured to remove or block the analyte from the composition; and 
 flowing the first portion of the composition over a surface of the sensor to equilibrate the sensor for testing the presence of an analyte in a second portion of the composition. 
 
     
     
       17. The method of  claim 16 , wherein the composition is introduced into a first sample compartment of a device configured to determine the presence of an analyte in the composition, and wherein the first portion of the composition is diverted to a first sub-compartment of the device. 
     
     
       18. The method of  claim 17 , wherein the first sub-compartment comprises the material configured to remove or block the analyte from the composition. 
     
     
       19. The method of  claim 17 , wherein contacting the first portion of the composition with the material configured to remove or block the analyte from the composition comprises flowing the first portion of the composition over or through the material. 
     
     
       20. The method of  claim 19 , wherein flowing the first portion of the composition over or through the material comprises flowing the first portion of the composition through a channel that is downstream from the first sub-compartment, wherein the material is disposed on a surface of the channel or disposed in the channel.