Transgenic betta

Abstract

The present invention relates to transgenic ornamental fish, as well as methods of making such fish by germ cell transplantation techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

What is claimed is: 
     
       1. A transgenic Betta comprising a chromosomally integrated expression cassette encoding a green, red or orange fluorescent protein, wherein the Betta comprises a transformation event from sperm selected from a group consisting of a “Green Betta 1 transformation event”, an “Orange Betta 1 transformation event and a “Red Betta 1 transformation event”, sperm exhibiting the Green Betta 1 transformation event having been deposited at the ATCC as deposit number PTA-126548, sperm exhibiting the Orange Betta 1 transformation event having been deposited at the ATCC as deposit number PTA-126549, and sperm exhibiting the Red Betta 1 transformation event having been deposited at the ATCC as deposit number PTA-126550. 
     
     
       2. The transgenic Betta of  claim 1 , further defined as a fertile, transgenic Betta. 
     
     
       3. The transgenic Betta of  claim 1 , wherein the fish is homozygous for the integrated expression cassette. 
     
     
       4. The transgenic Betta of  claim 1 , wherein the fish is heterozygous for the integrated expression cassette. 
     
     
       5. A method of providing a transgenic Betta to the ornamental fish market, comprising obtaining a transgenic Betta in accordance with  claim 1 , and distributing the fish to the ornamental fish market. 
     
     
       6. The method of  claim 5 , wherein the fish are distributed by a grower to a commercial distributor. 
     
     
       7. The method of  claim 5 , wherein the fish are distributed by a grower or a commercial distributor to a retailer. 
     
     
       8. The method of  claim 7 , wherein the retailer is a multi-product retailer having an ornamental fish department. 
     
     
       9. A method of producing a transgenic Betta comprising: (a) obtaining a Betta that comprises a chromosomally integrated expression cassette encoding a green, red or orange fluorescent protein in accordance with  claim 1 ; and (b) breeding the obtained Betta with a second Betta to provide a transgenic Betta, wherein the transgenic Betta comprises the transformation event. 
     
     
       10. The method of  claim 9 , wherein the second Betta is a non-transgenic Betta. 
     
     
       11. A progeny of a transgenic Betta of  claim 1  that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the progeny Betta comprises a transformation event selected from a group consisting of a “Green Betta 1 transformation event”, an “Orange Betta 1 transformation event”, a “Red Betta 1 transformation event”,. 
     
     
       12. The progeny fish of  claim 11 , further defined as a fertile, transgenic Betta. 
     
     
       13. The progeny fish of  claim 11 , wherein the progeny Betta is homozygous for the integrated expression cassette. 
     
     
       14. The progeny fish of  claim 11 , wherein the progeny Betta is heterozygous for the integrated expression cassette. 
     
     
       15. A method of providing a transgenic fish to the ornamental fish market, comprising obtaining a progeny fish in accordance with  claim 11 , and distributing the fish to the ornamental fish market. 
     
     
       16. The method of  claim 15 , wherein the fish are distributed by a grower to a commercial distributor. 
     
     
       17. The method of  claim 16 , wherein the fish are distributed by a grower or a commercial distributor to a retailer. 
     
     
       18. The method of  claim 17 , wherein the retailer is a multi-product retailer having an ornamental fish department. 
     
     
       19. A method of producing a transgenic fish comprising: (a) obtaining a transgenic fish in accordance with  claim 11 ; and (b) breeding the obtained fish with a second fish to provide a transgenic fish comprising the transformation event. 
     
     
       20. The method of  claim 19 , wherein the second fish is a non-transgenic fish. 
     
     
       21. Cell lines for making a transgenic Betta comprising: cells comprising the Green Betta 1 transformation event deposited at the ATCC as deposit number PTA-126548, cells comprising the Orange Betta 1 transformation event deposited at the ATCC as deposit number PTA-126549, and cells comprising the Red Betta 1 transformation event deposited at the ATCC as deposit number PTA-126550.