Devices for cultivating cells coated with a copolymer of polyethyleneglykol (meth)acrylate

Abstract

Device D for cultivating cell cultures C, wherein said device D has a surface O and comprises on its surface O at least one polymer P wherein said polymer P is a copolymer of at least one monomer M and at least one ester E of (meth)acrylic acid and polyethylene oxide, wherein said monomer M is different from ester E and has at least one ethylenically unsaturated double bond.

The invention claimed is: 
     
       1. A device D for cultivating non-adherent cell cultures C, wherein said device D has a surface O and comprises on its surface O at least one polymer P,
 wherein said polymer P is a copolymer of styrene and at least one ester E of (meth)acrylic acid and polyethylene oxide; 
 wherein the device D comprises free floating non-adherent cells. 
 
     
     
       2. The device D according to  claim 1 , wherein said device D is selected from the group consisting of flasks, cell culture dishes, cell culture bottles, cell culture bags, pipette tips, petri dishes, multiwall plates, reactors, tubes, pipettes, and syringes. 
     
     
       3. The device D according to  claim 1 , wherein the polymer P comprises styrene and the at least one ester E in a molar ratio of 0.3:1 to 10:1. 
     
     
       4. The device D according to  claim 1 , wherein the polymer P has a surface adsorption SA of at least 200 ng/cm 2  on the respective surface where polymer P is deposited, said SA being determined by quartz crystal microbalance. 
     
     
       5. The device D according to  claim 1 , wherein the at least one ester E has an average molar mass Mn of 300 to 10,000 g/mol. 
     
     
       6. The device D according to  claim 1 , wherein the polymer P has an average molar mass Mn of 5000 to 100,000 g/mol. 
     
     
       7. The device D according to  claim 1 , wherein the surface O comprises glass, quartz, silicon, metals, metal oxides or organic polymers. 
     
     
       8. The device D according to  claim 1 , wherein the surface O comprises fluorinated polyolefins, or partially fluorinated polyolefins. 
     
     
       9. A process for cultivating cell cultures C, comprising:
 a) providing the device D suitable for cultivating cell cultures according to  claim 1 ; and 
 b) cultivating non-adherent cell cultures C in the supernatant medium above the surface of the device D. 
 
     
     
       10. The device D according to  claim 1 , wherein the polymer P is a statistical copolymer in which styrene and ester E are distributed statistically. 
     
     
       11. The device D according to  claim 1 , wherein said polymer P has a surface adsorption SA of at least 200 ng/cm 2  on the respective surface where polymer P is deposited. 
     
     
       12. The device D according to  claim 1 , wherein the non-adherent cell cultures C are not attached to the surface O.