Albumin-based non-covalent complexes and methods of use thereof

Abstract

A non-covalent complex of an albumin molecule and a hydrophobic ligand, compositions containing the same, and methods of use thereof are provided. The present complex may find use in delivering the hydrophobic ligand to microorganisms that have albumin-binding outer surfaces, such as a cell wall.

What is claimed is: 
     
       1. A method for determining antimicrobial susceptibility of a microorganism for an antimicrobial, the method comprising:
 contacting a composition comprising a microorganism comprising a cell wall with the antimicrobial; 
 contacting the composition comprising the microorganism and the antimicrobial with an aqueous solution comprising a non-covalent complex of a hydrophobic molecule and a single albumin protein, wherein the hydrophobic molecule functionally associates with the microorganism; 
 detecting one or more properties of the hydrophobic molecule in the composition; and 
 determining the antimicrobial susceptibility of the microorganism for the antimicrobial based on the detected property of the hydrophobic molecule. 
 
     
     
       2. The method of  claim 1 , wherein the microorganism is a pathogenic microorganism. 
     
     
       3. The method of  claim 1 , wherein contacting of the composition comprising the microorganism with the antimicrobial occurs in vitro. 
     
     
       4. The method of  claim 1 , wherein the hydrophobic molecule is a carotenoid. 
     
     
       5. The method of  claim 4 , wherein the carotenoid is a carotene. 
     
     
       6. The method of  claim 5 , wherein the carotene is lycopene or β-carotene. 
     
     
       7. The method of  claim 1 , wherein the antimicrobial is an antibacterial. 
     
     
       8. The method of  claim 1 , wherein the antimicrobial is an antifungal. 
     
     
       9. The method of  claim 1 , wherein the albumin protein is a human serum albumin protein. 
     
     
       10. The method of  claim 1 , wherein the method further comprises forming the aqueous solution comprising the non-covalent complex of hydrophobic molecule and single albumin protein by:
 dissolving the hydrophobic molecule in:
 i) a first organic solvent comprising a C 3 -C 5  ketone; or 
 ii) a combination of the first organic solvent and a second organic solvent in a ratio of from about 0.001:1 to about 1000:1 v/V, to provide a first solution; 
 
 combining the first solution with a second solution to provide a third solution, wherein the second solution is an aqueous solution comprising an albumin protein; and 
 removing the first organic solvent or the combination of the first organic solvent and the second organic solvent from the third solution to provide a fourth solution, wherein the fourth solution is an aqueous solution comprising a non-covalent complex of the hydrophobic molecule and a single albumin protein. 
 
     
     
       11. The method of  claim 10 , wherein the C 3 -C 5  ketone is acetone. 
     
     
       12. The method of  claim 10 , wherein the hydrophobic molecule is dissolved in the combination of a first organic solvent and a second organic solvent, and wherein the ratio of the first organic solvent and the second organic solvent is in the range of about 1:1 to about 5:1. 
     
     
       13. The method of  claim 12 , wherein the hydrophobic molecule is dissolved in the combination, and wherein the combination comprises the first organic solvent and the second organic solvent in a ratio of about 2:1. 
     
     
       14. The method of  claim 10 , wherein the hydrophobic molecule is dissolved in the combination, and wherein the method comprises:
 dissolving the hydrophobic molecule in the second organic solvent, to provide a fifth solution; and 
 combining the fifth solution with the C 3 -C 5  ketone to provide the first solution prior to combining the first solution with the second solution. 
 
     
     
       15. The method of  claim 10 , wherein the removing is performed by evaporation.