US12442024B2ActiveUtilityA1

Dihomo-gamma-linolenic acid-containing microbial oil/lipid with reduced arachidonic acid content

Assignee: NIPPON SUISAN KAISHA LTDPriority: Dec 28, 2018Filed: Jun 28, 2021Granted: Oct 14, 2025

Est. expiryDec 28, 2038(~12.5 yrs left)· nominal 20-yr term from priority

Inventors:Seizo Sato Takayoshi Sekiguchi Misaki Takahashi

C12Y 114/19003C12N 9/0071A61K 45/06A61K 31/202C12N 1/145C12R 2001/645A61P 31/02C12P 7/6418C12P 7/6427A61K 2800/10A61K 8/99A61K 2800/86A61K 8/92A61K 8/361A61K 35/66A23D 9/00A23V 2250/1874A23V 2300/14C11C 3/003A61K 31/201A61K 31/20A61Q 19/00A61P 37/08A61P 29/00A61K 36/06A61K 31/232A61K 8/37C11B 1/10

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Claims

Abstract

The purpose of the present invention is to provide an oil/lipid that contains dihomo-γ-linolenic acid at a higher purity. Provided is a microbial oil/lipid that is specified by a high content of dihomo-γ-linolenic acid contained therein and/or a reduced content of undesirable constituting fatty acid(s).

Claims

exact text as granted — not AI-modified

The invention claimed is: 
     
       1. A microbial oil/lipid comprising dihomo-γ-linolenic acid and at least one of C24:0, C22:0, and C20:0 as constituent fatty acids, wherein the microbial oil/lipid comprises (i) dihomo-γ-linolenic acid in a content of greater than 30 wt. %, greater than 32 wt. %, or greater than 34 wt. %; (ii) C24:0, C22:0, and C20:0, in a total content of 12.0 wt. % or less, 11.0 wt. % or less, 10.0 wt. % or less, 9.0 wt. % or less, or 8.0 wt. % or less; and (iii) substantially no arachidonic acid or arachidonic acid in a content of 0.3 wt. % or less, 0.2 wt. % or less, 0.1 wt. % or less, 0.05 wt. % or less, 0.04 wt. % or less, 0.03 wt. % or less, or 0.01 wt. % or less, wherein the microbial oil/lipid comprises no Δ5 desaturase inhibitor. 
     
     
       2. The microbial oil/lipid according to  claim 1 , wherein as the constituent fatty acid of the oil, a content of C24:0 is 8.5 wt. % or less, 7.0 wt. % or less, 6.0 wt. % or less, or 5.5 wt. % or less. 
     
     
       3. The microbial oil/lipid according to  claim 1 , wherein as the constituent fatty acid of the oil, a total content of C24:0 and C22:0 is 11.0 wt. % or less, 10.0 wt. % or less, 9.0 wt. % or less, 8.0 wt. % or less, or 7.5 wt. % or less. 
     
     
       4. The microbial oil/lipid according to  claim 1 , wherein a compositional ratio of arachidonic acid to the dihomo-γ-linolenic acid as the constituent fatty acid of the oil is 1/120 or less, 1/180 or less, 1/360 or less, 1/1000 or less, 1/2350 or less, or 1/3000 or less in terms of weight ratio. 
     
     
       5. The microbial oil/lipid according to  claim 4 , wherein a compositional ratio of C24:0 to the dihomo-γ-linolenic acid as the constituent fatty acid of the oil is 1/4.1 or less, 1/5.0 or less, or 1/6.0 or less in terms of weight ratio. 
     
     
       6. The microbial oil/lipid according to  claim 4 , wherein a compositional ratio of a total of C24:0 and C22:0 to the dihomo-γ-linolenic acid as the constituent fatty acid of the oil is 1/3.1 or less, 1/4.0 or less, or 1/5.0 or less in terms of weight ratio. 
     
     
       7. The microbial oil/lipid according to  claim 4 , wherein a compositional ratio of a total of C24:0, C22:0, and C20:0 to the dihomo-γ-linolenic acid as the constituent fatty acid of the oil is 1/3.0 or less, 1/3.9 or less, or 1/4.8 or less in terms of weight ratio. 
     
     
       8. The microbial oil/lipid according to  claim 1 , wherein a compositional ratio of C24:0 to the dihomo-γ-linolenic acid as the constituent fatty acid of the oil is 1/4.1 or less, 1/5.0 or less, or 1/6.0 or less in terms of weight ratio. 
     
     
       9. The microbial oil/lipid according to  claim 8 , wherein a compositional ratio of a total of the C24:0 and C22:0 to the dihomo-γ-linolenic acid as the constituent fatty acid of the oil is 1/3.1 or less, 1/4.0 or less, or 1/5.0 or less in terms of weight ratio. 
     
     
       10. The microbial oil/lipid according to  claim 8 , wherein a compositional ratio of a total of the C24:0, C22:0, and C20:0 to the dihomo-γ-linolenic acid as the constituent fatty acid of the oil is 1/3.0 or less, 1/3.9 or less, or 1/4.8 or less in terms of weight ratio. 
     
     
       11. The microbial oil/lipid according to  claim 1 , wherein a compositional ratio of a total of C24:0 and C22:0 to the dihomo-γ-linolenic acid as the constituent fatty acid of the oil is 1/3.1 or less, 1/4.0 or less, or 1/5.0 or less in terms of weight ratio. 
     
     
       12. The microbial oil/lipid according to  claim 11 , wherein a compositional ratio of a total of the C24:0, the C22:0, and C20:0 to the dihomo-γ-linolenic acid as the constituent fatty acid of the oil is 1/3.0 or less, 1/3.9 or less, or 1/4.8 or less in terms of weight ratio. 
     
     
       13. The microbial oil/lipid according to  claim 1 , wherein a compositional ratio of a total of C24:0, C22:0, and C20:0 to the dihomo-γ-linolenic acid as the constituent fatty acid of the oil is 1/3.0 or less, 1/3.9 or less, or 1/4.8 or less in terms of weight ratio. 
     
     
       14. The microbial oil/lipid according  claim 1 , wherein the microbial oil/lipid is an oil derived from a filamentous fungus. 
     
     
       15. The microbial oil/lipid according to  claim 14 , wherein the filamentous fungus belongs to genus  Mortierella.    
     
     
       16. A microbial oil/lipid comprising the microbial oil/lipid described in  claim 1 , wherein the microbial oil/lipid is a crude oil. 
     
     
       17. A pharmaceutical composition comprising the microbial oil/lipid described in  claim 1 . 
     
     
       18. An anti-allergic agent or an anti-inflammatory agent comprising the microbial oil/lipid described in  claim 1 . 
     
     
       19. A lower alcohol ester composition or a free fatty acid composition comprising dihomo-γ-linolenic acid, obtained or obtainable by a method comprising providing the microbial oil/lipid described in  claim 1  to an ester exchange reaction or a hydrolysis reaction. 
     
     
       20. The composition according to  claim 19 , comprising the dihomo-γ-linolenic acid in a content of greater than 30 wt. %, greater than 32 wt. %, or greater than 34 wt. %. 
     
     
       21. The composition according to  claim 19 , comprising substantially no arachidonic acid or comprising arachidonic acid in a content of 0.3 wt. % or less, 0.2 wt. % or less, 0.1 wt. % or less, 0.05 wt. % or less, 0.04 wt. % or less, 0.03 wt. % or less, or 0.01 wt. % or less. 
     
     
       22. The composition according to  claim 19 , wherein a content of C24:0 is 8.5 wt. % or less, 7.0 wt. % or less, 6.0 wt. % or less, or 5.5 wt. % or less. 
     
     
       23. The composition according to  claim 19 , wherein a total content of C24:0 and C22:0 is 11.0 wt. % or less, 10.0 wt. % or less, 9.0 wt. % or less, 8.0 wt. % or less, or 7.5 wt. % or less. 
     
     
       24. The composition according to  claim 19 , wherein a total content of C24:0, C22:0, and C20:0 is 12.0 wt. % or less, 11.0 wt. % or less, 10.0 wt. % or less, 9.0 wt. % or less, or 8.0 wt. % or less. 
     
     
       25. The composition according to  claim 19 , wherein a compositional ratio of arachidonic acid to the dihomo-γ-linolenic acid as a constituent fatty acid of an oil is 1/120 or less, 1/180 or less, 1/360 or less, 1/1000 or less, 1/2350 or less, or 1/3000 or less in terms of weight ratio. 
     
     
       26. The composition according to  claim 19 , wherein a compositional ratio of C24:0 to the dihomo-γ-linolenic acid as a constituent fatty acid of an oil is 1/4.1 or less, 1/5.0 or less, or 1/6.0 or less in terms of weight ratio. 
     
     
       27. The composition according to  claim 19 , wherein a compositional ratio of a total of C24:0 and C22:0 to the dihomo-γ-linolenic acid as a constituent fatty acid of an oil is 1/3.1 or less, 1/4.0 or less, or 1/5.0 or less in terms of weight ratio. 
     
     
       28. The composition according to  claim 19 , wherein a compositional ratio of a total of C24:0, C22:0, and C20:0 to the dihomo-γ-linolenic acid as a constituent fatty acid of an oil is 1/3.0 or less, 1/3.9 or less, or 1/4.8 or less in terms of weight ratio. 
     
     
       29. A microbial biomass comprising the microbial oil/lipid described in  claim 1 . 
     
     
       30. A foodstuff, a supplement, a medicament, a cosmetic, or an animal feed comprising the microbial biomass described in  claim 29 . 
     
     
       31. A culture comprising the microbial biomass described in  claim 29 . 
     
     
       32. The culture according to  claim 31 , comprising the microbial oil/lipid in a content of 0.4 g/liter or greater. 
     
     
       33. A method of producing the microbial oil/lipid according to  claim 1 , the method comprising culturing, in a culture medium, a microorganism strain having a mutation in a Δ5 desaturase gene to produce the dihomo-γ-linolenic acid-containing microbial oil/lipid. 
     
     
       34. The method according to  claim 33 , wherein the microorganism strain has a gene mutation that results in an amino acid substitution of Y310C of SEQ ID NO: 3 of a Δ5 desaturase. 
     
     
       35. The method according to  claim 33 , wherein the microorganism strain has a base deletion at positions from 2206 to 2234 of SEQ ID NO: 1 of the Δ5 desaturase gene. 
     
     
       36. The method according to  claim 33 , wherein the microorganism strain belongs to genus  Mortierella.    
     
     
       37. The method according to  claim 33 , wherein the microorganism strain is a microorganism deposited under accession number NITE BP-02778. 
     
     
       38. A microorganism having deposited under accession number NITE BP-02778.

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