Recombinant antibacterial peptide TRSUB, preparation method and application for same

Abstract

A recombinant antibacterial peptide TrSub, preparation method and application for the recombinant antibacterial peptide TrSub is disclosed, and belongs to the field of genetic engineering and biotechnology. The amino acid sequence of the recombinant antimicrobial peptide TrSub is shown in SEQ ID No. 1. The nucleotide sequence encoding the amino acid shown in SEQ ID NO.1 is shown in SEQ ID NO.2. The disclosure also provides a method for preparing the recombinant antimicrobial peptide TrSub, wherein the recombinant antimicrobial peptide TrSub has an inhibitory effect on Escherichia coli, Salmonella, Staphylococcus aureus and Clostridium perfringens . The disclosure has good thermal stability, acid resistance, pepsin resistance and low hemolytic activity, and is beneficial to the application of the disclosure in the preparation of fee and feed additive.

What is claimed is: 
     
         1 . A recombinant antibacterial peptide TrSub an having the amino acid sequence shown in SEQ ID NO.1. 
     
     
         2 . A synthetic nucleotide sequence encoding the amino acid shown in SEQ ID NO.1, wherein the nucleotide sequence is shown in SEQ ID NO.2. 
     
     
         3 . A recombinant expression vector comprising the synthetic nucleotide sequence shown in SEQ ID NO.2. 
     
     
         4 . A recombinant host strain comprising the synthetic nucleotide sequence shown in SEQ ID NO.2, wherein said recombinant host strain is  Trichoderma reesei  Tu6. 
     
     
         5 . A product comprising the recombinant antibacterial peptide TrSub of  claim 1 , wherein said product is a feed or its additives.