Lactiplantibacillus plantarum GOLDGUT-LP618 having function of resisting salmonella infection and application thereof

Abstract

The present invention relates to the technical field of probiotics, in particular to a Lactiplantibacillus plantarum GOLDGUT-LP618 having a function of resisting Salmonella infection and application thereof. The Lactiplantibacillus plantarum GOLDGUT-LP618 provided by the present invention is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 28399. The strain has high resistance to acid and bile salt and can adapt to the digestive tract environment. The strain can inhibit Salmonella infection, relieve diarrhea, inflammation and intestinal damage caused by Salmonella infection, and promote intestinal health. The strain can be used for preparing functional foods, dietary supplements or drugs that can resist Salmonella infection and improve diarrhea and inflammation caused by Salmonella infection, and has good application prospects.

What is claimed is: 
     
         1 . A method of preparing a microbial preparation for resisting  Salmonella  infection, wherein the microbial preparation comprising a Lactiplantibacillus  plantarum  GOLDGUT-LP618 strain, which was deposited at the China General Microbiological Culture Collection Center (CGMCC), with a deposition number of CGMCC No. 28399;
 wherein the method comprising steps:   (a) inoculating the Lactiplantibacillus  plantarum  GOLDGUT-LP618 strain into an MRS liquid medium to form a mixture;   (b) incubating the mixture at 37° C. to obtain an enriched solution;   (c) performing a tenfold gradient on the enrichment solution to generate dilutions with gradient concentrations of 10 −5 , 10 −6 , and 10 −7 ;   (d) applying 100 μL of each of the dilutions to sterile a first MRS solid medium and incubating under aerobic conditions at 37° C. for 24 to 48 hours, then single colonies are formed;   (e) selecting the single colonies; and   (f) repeatedly streaking the selected colonies on a second MRS solid medium for purification to obtain a purified strain.