Pesticidal genes and methods of use

Abstract

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Methods are provided for producing the polypeptides disclosed herein, and for using those polypeptides for controlling a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

That which is claimed is: 
     
         1 . A polypeptide comprising:
 (a) an amino acid sequence having at least 96% sequence identity to the amino acid sequence set forth in SEQ ID NO: 186 or 185, wherein the polypeptide has pesticidal activity, and wherein the polypeptide further comprises a chemically linked heterologous amino acid sequence; or   (b) the amino acid sequence set forth in SEQ ID NO: 186 or 185, wherein the polypeptide further comprises a chemically linked heterologous amino acid sequence.   
     
     
         2 . A recombinant nucleic acid molecule encoding a polypeptide comprising:
 (a) an amino acid sequence having at least 96% sequence identity to the amino acid sequence set forth in SEQ ID NO: 186 or 185, wherein the polypeptide has pesticidal activity, and wherein the recombinant nucleic acid molecule is operably linked to a heterologous promoter; or   (b) the amino acid sequence set forth in SEQ ID NO: 186 or 185, wherein the recombinant nucleic acid molecule is operably linked to a heterologous promoter.   
     
     
         3 . The nucleic acid of  claim 2 , wherein said nucleic acid molecule is a synthetic sequence designed for expression in a plant. 
     
     
         4 . A host cell comprising the nucleic acid molecule of  claim 2 . 
     
     
         5 . A DNA construct comprising a heterologous promoter operably linked to a nucleotide sequence that encodes a polypeptide comprising:
 (a) an amino acid sequence having at least 96% sequence identity to the amino acid sequence set forth in SEQ ID NO: 186 or 185, wherein the polypeptide has pesticidal activity; or   (b) the amino acid sequence set forth in SEQ ID NO: 186 or 185.   
     
     
         6 . The DNA construct of  claim 5 , wherein the promoter drives expression in a plant cell or a bacterial cell. 
     
     
         7 . The DNA construct of  claim 5 , wherein said nucleotide sequence is a synthetic DNA sequence designed for expression in a plant. 
     
     
         8 . A vector comprising the DNA construct of  claim 5 . 
     
     
         9 . A host cell comprising the DNA construct of  claim 5 . 
     
     
         10 . A formulation comprising the polypeptide of  claim 1 . 
     
     
         11 . A method for controlling a pest population, the method comprising contacting said pest population with a pesticidal-effective amount of the formulation of  claim 10 . 
     
     
         12 . A method for producing a polypeptide with pesticidal activity, the method comprising culturing the host cell of  claim 9  under conditions in which the nucleic acid molecule encoding the polypeptide is expressed. 
     
     
         13 . A plant having stably incorporated into its genome a DNA construct comprising the nucleic acid molecule of  claim 2 . 
     
     
         14 . A transgenic seed of the plant of  claim 13 , wherein said seed has stably incorporated into its genome the DNA construct. 
     
     
         15 . The plant of  claim 13 , wherein the plant is a monocot or a dicot. 
     
     
         16 . The plant of  claim 15 , wherein
 (a) the monocot plant is corn, sorghum, wheat, rice, sugarcane, barley, oats, rye, millet, coconut, pineapple or banana; or   (b) the dicot plant is sunflower, tomato, crucifers, peppers, potato, cotton, soybean, sugarbeet, tobacco, oilseed rape, sweet potato, alfalfa, safflower, peanuts, cassava, coffee, cocoa, cucumber, lettuce, olive, peas, or tea.   
     
     
         17 . A method for protecting a plant from an insect pest, the method comprising expressing in a plant or cell thereof the nucleic acid molecule of  claim 2 . 
     
     
         18 . The method of  claim 17 , wherein protecting said plant comprises controlling insect pest damage to said plant. 
     
     
         19 . A method for increasing yield in a plant, the method comprising growing in a field a plant or seed thereof having stably incorporated into its genome a DNA construct comprising a promoter that drives expression in a plant operably linked to the nucleic acid molecule of  claim 2 .