Fat and/or wax activated by means of the water-insoluble fraction of Carica papaya sap

Abstract

The invention relates to a method for obtaining the water-insoluble fraction of Carica papaya sap, enriched with Carica papaya lipase, the water-insoluble fraction obtainable by this method, a method for the preparation of an activated fat and/or an activated wax by means of said water-insoluble fraction of the Carica papaya sap, the activated fat and/or the activated wax capable of being obtained by this method, a composition combining said activated fat and/or said activated wax, as well as the cosmetic use of these products.

What is claimed is: 
     
         1 . A method for enriching a fat with diacylglycerols and/or a fatty alcohol-enriched wax, the method comprising:
 1) obtaining a water-insoluble fraction of  Carica papaya  sap, enriched with  Carica papaya  lipase, by:
 a) suspending dried  Carica papaya  sap in distilled water in a weight ratio of dried raw  Carica papaya /distilled water of between 0.01 and 0.5, between 0.05 and 0.25, between 0.08 and 0.2, or 0.1 to create a suspension; 
 b) stirring the suspension obtained in step a) for a time period of between 15 minutes and 240 minutes, a time period of between 30 minutes and 180 minutes, between 60 minutes and 150 minutes, or 120 minutes at room temperature; 
 c) centrifuging the suspension obtained in step b) for a time period of between 5 minutes and 90 minutes, between 15 minutes and 60 minutes, or between 20 and 40 minutes, and at a rotation speed of between 2000 and 6000 rpm, between 3000 and 5000 rpm, or at 4000 rpm to obtain a pellet containing the water-insoluble fraction of the  Carica papaya  sap, enriched with  Carica papaya  lipase; and 
 d) recovering the pellet containing the water-insoluble fraction of the  Carica papaya  sap, enriched with  Carica papaya  lipase from the suspension; and 
   2) providing a fat and/or wax; and   3) combining the water-insoluble fraction of the  Carica papaya  sap, enriched with  Carica papaya  lipase, from step d) to the fat and/or wax, to produce the diacylglycerol-enriched fat and/or the fatty alcohol-enriched wax.   
     
     
         2 . The method of  claim 1 , the method further comprising, after separating the pellet from the suspension, drying the pellet until a particulate powder of the water-insoluble fraction is produced and then adding the particular powder to the fat and/or wax to produce the diacylglycerol-enriched fat and/or the fatty alcohol-enriched wax. 
     
     
         3 . The method of  claim 1 , wherein the method is directed to the preparation of diacylglycerol-enriched fats, and wherein the method step  2 ) further comprises:
 i) mixing the fat with an amount of water or with an amount of saline solution in a volume ratio of water or saline solution to fat of between 0.005 and 0.5, or between 0.01 and 0.4 to produce an aqueous mixture; and   ii) keeping the aqueous mixture of step i) under agitation at a temperature of between 30° C. and 70° C., for a time period of between 1 and 30 minutes to produce an emulsion.   
     
     
         4 . The method of  claim 3 , wherein step  3 ) further comprises:
 iii) while still under agitation and temperature, combining the emulsion of fat and water from step ii) with a given volume of the water-insoluble fraction of the  Carica papaya  sap, enriched with  Carica papaya  lipase, from step d) in a volume ratio of water-insoluble fraction of the  Carica papaya  sap to fat of between 0.01 and 0.2; and   iv) maintaining the temperature and agitation for a time period of between one hour to six hours, between two and five hours, or about four hours, to obtain a mixture of the diacylglycerol-enriched fat, a portion of the water-insoluble fraction of  Carica papaya  sap, enriched in  Carica papaya  lipase, and a reaction medium.   
     
     
         5 . The method of  claim 4 , further comprising:
 v) separating the portion of water-insoluble fraction of  Carica papaya  sap, enriched in  Carica papaya  lipase, from the reaction medium containing the diacylglycerol-enriched fat using at least one gradient filter or at least one superposition of at least two filters of decreasing porosity, from around 500 μm to around 250 μm.   
     
     
         6 . The method of  claim 5 , wherein the method further comprises at least one of the following two steps:
 v′) removing at least a portion of residual water present in the reaction medium with a drying agent, and/or   v″) deodorizing and/or improving the shine of the diacylglycerol-enriched fat with activated carbon.   
     
     
         7 . The method according to  claim 3 , wherein the fat is an animal, plant, and/or marine origin oil, and is virgin or refined. 
     
     
         8 . The method of  claim 3 , wherein the fat is an oil comprising triglycerides with aliphatic hydrocarbon chains of a fatty acid having a carbon number of between 12 and 26, between 16 and 20, or between 16 and 18; the aliphatic hydrocarbon chain being linear or branched, saturated or unsaturated, and when unsaturated, having a number of unsaturations of between 1 and 6, or between 1 and 3. 
     
     
         9 . The method of  claim 3 , wherein the fat is an oil comprising mono- or polyhydroxylated, and/or mono- or polymethoxylated, and/or mono- or polyoxidized, and/or mono- or poly-epoxylated chains. 
     
     
         10 . The method of  claim 3 , wherein the fat is an oil selected from calophyllum inophyllum oil, raspberry oil, camellia oil, evening primrose oil, Brazil nut oil, baobab oil and olive oil, or a mixture of at least two of these oils. 
     
     
         11 . The method of  claim 3 , wherein the diacylglycerol-enriched fat comprises diacylglycerols that consist of 1,2-diacylglycerols and 1,3-diacylglycerols, respectively represented by the following formulas (I) and (II): 
       
         
           
           
               
               
           
         
         wherein R1 and R2 are:
 aliphatic hydrocarbon chains of a fatty acid having a carbon number of between 12 and 26, between 16 and 20, or between 16 and 18; said aliphatic hydrocarbon chain being linear or branched, saturated or unsaturated, and when unsaturated, with a number of unsaturations of between 1 and 6, or between 1 and 3; or 
 mono- or polyhydroxylated, and/or mono- or polymethoxylated, and/or mono- or polyoxidized, and/or mono- or poly-epoxylated chains. 
 
       
     
     
         12 . The method of  claim 3 , wherein the diacylglycerol-enriched fat comprises diacylglycerols that consist essentially of, 1,2-diacylglycerols represented by the following formula (I): 
       
         
           
           
               
               
           
         
         wherein R1 and R2 are:
 aliphatic hydrocarbon chains of a fatty acid having a carbon number of between 12 and 26, between 16 and 20, or between 16 and 18; said aliphatic hydrocarbon chain being linear or branched, saturated or unsaturated, and when unsaturated, with a number of unsaturations of between 1 and 6, or between 1 and 3; or 
 mono- or polyhydroxylated, and/or mono- or polymethoxylated, and/or mono- or polyoxidized, and/or mono- or poly-epoxylated chains.