Cell-coated supports

Abstract

Devices and methods for enhancing the healing of wounds, especially chronic wounds (e.g., diabetic wounds), involving the use of keratinocytes and transformed cells are described. A cell-coated transplantable solid support (e.g., beads coated with keratinocytes and/or transformed cells), are placed in an enclosure. The enclosure, in turn, is placed in the wound for use as an interactive wound healing promoter.

We claim:  
     
         1 . A system for the treatment of wounds, comprising: 
 a) transformed cells on a solid support;    b) an enclosure, said enclosure housing said solid support; and    c) means for removing said enclosure from a wound.    
     
     
         2 . The system of    claim 1   , wherein said solid support comprises beads.  
     
     
         3 . The system of    claim 2   , wherein said beads are macroporous.  
     
     
         4 . The system of    claim 3   , wherein said beads are polyethylene.  
     
     
         5 . The system of    claim 1   , wherein said enclosure comprises a mesh material, having pores.  
     
     
         6 . The system of    claim 5   , wherein said mesh material comprises polyethylene.  
     
     
         7 . The system of    claim 1   , wherein said enclosure comprises a biocompatible membrane.  
     
     
         8 . The system of    claim 1   , wherein said transformed cells are selected from the group consisting of fibroblasts, keratinocytes, endothelial cells, melanocytes, smooth muscle cells, fetal fibroblasts, epithelial cells and combinations thereof.  
     
     
         9 . The system of    claim 1   , wherein said removal means comprises a string attached to said enclosure.  
     
     
         10 . The system of    claim 1   , wherein said transformed cells are cells transfected with a gene encoding a protein selected from the group consisting of EGF, VEGF, FGF, PDGF, IGF, KGF, IFN-α, IFN-δ, MSH, TGF-α, TGF-β, TNF-α, IL-1 and IL-6.  
     
     
         11 . A method for treating a wound, comprising the step of positioning the system of    claim 1    in a wound under conditions such that the healing of the wound is promoted.  
     
     
         12 . A method for treating a wound, comprising: 
 a) providing: i) transformed cells on a solid support, ii) an enclosure comprising mesh material having pores, and iii) a subject having at least one wound;    b) placing said transformed cell-containing solid support into said enclosure so as to produce a transformed cell-containing enclosure;    c) sealing said enclosure to produce a sealed transformed cell-containing enclosure;    d) positioning said sealed transformed cell-containing enclosure in the wound of said subject under conditions such that the healing of the wound is promoted; and    e) removing said sealed transformed cell-containing enclosure from said wound after healing is promoted.    
     
     
         13 . The method of    claim 12   , wherein said solid support is beads and said beads are mancroporous.  
     
     
         14 . The method of    claim 13   , wherein said beads are polyethylene.  
     
     
         15 . The method of    claim 13   , wherein said mesh material comprises polyethylene.  
     
     
         16 . The method of    claim 13   , wherein said enclosure comprises a biocompatible membrane.  
     
     
         17 . The method of    claim 13   , further comprising covering said sealed transformed cell-containing enclosure after positioning in step (c) in said wound with a dressing, and removing said dressing with said sealed transformed cell-containing enclosure in step (c).  
     
     
         18 . The method of    claim 12   , wherein said transformed cells are cells transfected with a gene encoding a protein selected from the group consisting of EGF, VEGF, FGF, PDGF, IGF, KGF, IFN-α, IFN-δ, MSH, TGF-α, TGF-β, TNF-α, IL-1 and IL-6.  
     
     
         19 . The method of    claim 12   , wherein said transformed cells are selected from the group consisting of fibroblasts, keratinocytes, endothelial cells, melanocytes, smooth muscle cells, fetal fibroblasts, epithelial cells and combinations thereof.