Method for inducing immunosuppressive cells and a culture device to be used therefor

Abstract

The present invention provides a method for inducing immunosuppressive cells by cultivating human cells with the use of a culture device having an affinity for protein and the culture device to be used therefor. According to the present invention, immunosuppressive cells can be efficiently induced by using a solid culture and can provide an efficient therapeutic system for diseases caused by hypersensitivity of the immune system with less side effect.

1 . A method for inducing immunosuppressive cells, which comprises culturing human cells with the use of a culture device having an affinity for protein.  
     
     
         2 . The inducing method of    claim 1   , wherein the culture device is previously coated with one or more cytokines or antibodies against surface antigens.  
     
     
         3 . The inducing method of    claim 1   , wherein the culture device is previously coated with two or more antibodies against surface antigens, each of said antibodies recognizing different epitope.  
     
     
         4 . The inducing method of    claim 1   , wherein human cells and one or more cytokines or antibodies against surface antigens are mixed.  
     
     
         5 . The inducing method of    claim 1   , wherein human cells and two or more antibodies against surface antigens, each of said antibodies recognizing different epitope, are mixed.  
     
     
         6 . The inducing method of    claim 2   ,    3   ,  4  or  5 , wherein the antibody against surface antigens is an anti-CD2 antibody or an anti-CD3 antibody.  
     
     
         7 . The inducing method of    claim 2   ,    3   ,  4 ,  5  or  6 , wherein the antibody against surface antigens is an anti-CD2 antibody, said anti-CD2 antibody binding to a site of CD2 that participates in the binding of LFA-3 to CD2.  
     
     
         8 . The inducing method of    claim 2   ,    3   ,  4 ,  5  or  6 , wherein the antibody against surface antigens is an anti-CD2 antibody, said anti-CD2 antibody binding to a site of CD2 other than a site of CD2 which participates in the binding of LFA-3 to CD2.  
     
     
         9 . The inducing method of    claim 3    or    5   , wherein two or more antibodies against surface antigens are anti-CD2 antibodies comprising a combination of one or more antibodies which bind to a site of CD2 that participates in the binding of LFA-3 to CD2 and one or more antibodies which bind to a site of CD2 other than the site of CD2 which participates in the binding of LFA-3 to CD2.  
     
     
         10 . The inducing method of    claim 2   ,    3   ,  4 ,  5 ,  6 ,  7  or  8 , wherein one antibody against surface antigens is the anti-CD2 antibody TS2/18.  
     
     
         11 . The inducing method of    claim 2   ,    3   ,  4 ,  5 ,  6 ,  7  or  8 , wherein one antibody against surface antigens is the F(ab)2 fragment of the anti-CD2 antibody TS2/18.  
     
     
         12 . The inducing method of    claim 2    or    4   , wherein the cytokine is IL-2 or GM-CSF.  
     
     
         13 . The inducing method of    claim 1   ,    2   ,  3 ,  4 ,  5 ,  6 ,  7 ,  8 ,  9 ,  10 ,  11  or  12 , wherein 0.5 to 10% by volume of serum based on a culture medium is mixed in the culture.  
     
     
         14 . The inducing method of    claim 1   ,    2   ,  3 ,  4 ,  5 ,  6 ,  7 ,  8 ,  9 ,  10 ,  11  or  12 , wherein no serum is mixed with the culture medium in the culture.  
     
     
         15 . The inducing method of    claim 1   ,    2   ,  3 ,  4 ,  5 ,  6 ,  7 ,  8 ,  9 ,  10 ,  11 ,  12 ,  13  or  14 , wherein a term of the culture ranges between 1 and 7 days.  
     
     
         16 . The inducing method of    claim 1   ,    2   ,  3 ,  4 ,  5 ,  6 ,  7 ,  8 ,  9 ,  10 ,  11 ,  12 ,  13 ,  14  or  15 , wherein the culture device is made of material having an affinity for protein.  
     
     
         17 . The inducing method of    claim 1   ,    2   ,  3 ,  4 ,  5 ,  6 ,  7 ,  8 ,  9 ,  10 ,  11 ,  12 ,  13 ,  14 ,  15  or  16 , wherein the culture device is made of plastic material.  
     
     
         18 . The inducing method of    claim 1   ,    2   ,  3 ,  4 ,  5 ,  6 ,  7 ,  8 ,  9 ,  10 ,  11 ,  12 ,  13 ,  14 ,  15  or  16 , wherein the culture device is made of glass material.  
     
     
         19 . The inducing method of    claim 1   ,    2   ,  3 ,  4 ,  5 ,  6 ,  7 ,  8 ,  9 ,  10 ,  11 ,  12 ,  13 ,  14 ,  15 ,  16 ,  17  or  18 , wherein the culture device is a closed plane plate vessel or a closed vessel charged with spherical fine-particles.  
     
     
         20 . A culture device having an affinity for protein, which is used for inducing immunosuppressive cells by culturing human cells.  
     
     
         21 . The culture device of    claim 20   , wherein the device is previously coated with one or more cytokines or antibodies against surface antigens.  
     
     
         22 . The culture device of    claim 20   , wherein the device is previously coated with two or more antibodies against surface antigens, each of said antibodies recognizing different epitope.  
     
     
         23 . The culture device of    claim 21    or    22   , wherein the antibody against surface antigens is anti-CD2 antibody or anti-CD3 antibody.  
     
     
         24 . The culture device of    claim 22    or    23   , wherein one or more antibodies that recognize different epitope are the anti-CD2 antibody TS2/18.