Novel glucosaminidase

Abstract

The invention provides glucosaminidase polypeptides and DNA (RNA) encoding glucosaminidase polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing glucosaminidase polypeptides to screen for antibacterial compounds.

What is claimed is:  
     
         1 . An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of: 
 (a) a polynucleotide having at least a 70% identity to a polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2;    (b) a polynucleotide having at least a 70% identity to a polynucleotide encoding the same mature polypeptide expressed by the glucosaminidase gene contained in the  Staphylococcus aureus  of the deposited strain;    (c) a polynucleotide encoding a polypeptide comprising an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID NO:2;    (d) a polynucleotide which is complementary to the polynucleotide of (a), (b) or (c); and    (e) a polynucleotide comprising at least 15 sequential bases of the polynucleotide of (a), (b), (c) or (d).    
     
     
         2 . The polynucleotide of    claim 1    wherein the polynucleotide is DNA.  
     
     
         3 . The polynucleotide of    claim 1    wherein the polynucleotide is RNA.  
     
     
         4 . The polynucleotide of    claim 2    comprising the nucleic acid sequence set forth in SEQ ID NO:1.  
     
     
         5 . The polynucleotide of    claim 2    comprising nucleotide 1 to 786 set forth in SEQ ID NO:1.  
     
     
         6 . The polynucleotide of    claim 2    which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2.  
     
     
         7 . A vector comprising the polynucleotide of    claim 1   .  
     
     
         8 . A host cell comprising the vector of    claim 7   .  
     
     
         9 . A process for producing a polypeptide comprising: expressing from the host cell of    claim 8    a polypeptide encoded by said DNA.  
     
     
         10 . A process for producing a glucosaminidase polypeptide or fragment comprising culturing a host of    claim 8    under conditions sufficient for the production of said polypeptide or fragment.  
     
     
         11 . A polypeptide comprising an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ I) NO:2.  
     
     
         12 . A polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:2.  
     
     
         13 . An antibody against the polypeptide of    claim 11   .  
     
     
         14 . An antagonist which inhibits the activity or expression of the polypeptide of    claim 11   .  
     
     
         15 . A method for the treatment of an individual in need of glucosaminidase polypeptide comprising: administering to the individual a therapeutically effective amount of the polypeptide of    claim 11   .  
     
     
         16 . A method for the treatment of an individual having need to inhibit glucosaminidase polypeptide comprising: administering to the individual a therapeutically effective amount of the antagonist of    claim 14   .  
     
     
         17 . A process for diagnosing a disease related to expression or activity of the polypeptide of    claim 11    in an individual comprising: 
 (a) determining a nucleic acid sequence encoding said polypeptide, and/or (b) analyzing for the presence or amount of said polypeptide in a sample derived from the individual.  
 
     
     
         18 . A method for identifying compounds which interact with and inhibit or activate an activity of the polypeptide of    claim 11    comprising: 
 contacting a composition comprising the polypeptide with the compound to be screened under conditions to permit interaction between the compound and the polypeptide to assess the interaction of a compound, such interaction being associated with a second component capable of providing a detectable signal in response to the interaction of the polypeptide with the compound;  
 and determining whether the compound interacts with and activates or inhibits an activity of the polypeptide by detecting the presence or absence of a signal generated from the interaction of the compound with the polypeptide.  
 
     
     
         19 . A method for inducing an immunological response in a mammal which comprises inoculating the mammal with glucosaminidase polypeptide of    claim 11   , or a fragment or variant thereof, adequate to produce antibody and/or T cell immune response to protect said animal from disease.  
     
     
         20 . A method of inducing immunological response in a mammal which comprises delivering a nucleic acid vector to direct expression of glucosaminidase polypeptide of    claim 11   , or fragment or a variant thereof, for expressing said glucosaminidase polypeptide, or a fragment or a variant thereof in vivo in order to induce an immunological response to produce antibody and/ or T cell immune response to protect said animal from disease.