Method for producing micro-carrier and test method by using said micro-carrier

Abstract

The invention provides a method for producing a microcarrier, which includes patterning pluralities of bar code on a mask; exposing the bar code to a substrate coated with photoresist; etching and removing residual photoresist and electroforming to a nickel plate; placing a bead coated with biotin or poly-L-lysine between two-nickel plates, and compressing the bar code on the surface of the bead to form a microcake-like particle with bar code; and combining the is particle with the corresponding bio-molecule thereof to produce a micro-carrier with a label. The invention also provides a test method for identifying a bio-molecule, which includes mixing several micro-carriers with the labeled unknown bio-molecules; and identifying the bar code on the micro-carrier via image recognition system, wherein the numbers and types of the known micro-carrier can be flexibly adjusted.

What is claimed is:  
     
         1 . A method for producing a micro-carrier of biomolecule, comprising: 
 preparing a bead and coating said bead with a layer of bio-molecule binding material;    patterning pluralities of bar code on a mask using an integrated circuit process, wherein the bar code represents the desired bio-molecule;    exposing the bar code to a substrate coated with photoresist using photolithography;    etching and removing residual photoresist, wherein the bar code is formed on the substrate, and electroforming to a nickel plate;    placing said bead between two-nickel plates, and compressing the bar code on the surface of said bead to form a microcake-like particle with a bar code;    coating a layer of bio-molecule binding material onto said particle; and    combining said particle with the corresponding biomolecule thereof to produce a micro-carrier with a label.    
     
     
         2 . The method as claimed in    claim 1   , wherein said particle is formed from the beads placed between two-nickel plates by dropping a UV photosenstizer micelle onto the nickel plates, followed by UV irradiation for curing.  
     
     
         3 . The method as claimed in    claim 1   , wherein said microcake-like particle is produced by simultaneously patterning a cake-like pattern and bar code on a mask, etching to form a mold, and molding by injection or hot compression.  
     
     
         4 . The method as claimed in    claim 1   , wherein said micro-carrier is further characterized by the shape, size, or color of said micro-carrier as a code.  
     
     
         5 . The method as claimed in    claim 1   , wherein the biomolecule comprises nucleic acid, oligonucleotide, peptide nucleic acid, antigen, antibody, enzyme or protein.  
     
     
         6 . The method as claimed in    claim 1   , wherein the biomolecule binding material comprises biotin or poly-L-lysine.  
     
     
         7 . A test method for identifying a bio-molecule, comprising: 
 providing a vial containing at least one micro-carrier with a bar code;    adding a labeled unknown bio-molecule to said vial and mixing, wherein a signal is obtained when said micro-carrier is complementary in combination with the unknown biomolecule;    transferring said micro-carrier in the vial onto a transporter, wherein a microscope connected with a computer is set above the transporter; and    identifying said bar code of the signaling microcarrier by an image recognition system, thereby identifying the unknown bio-molecule.    
     
     
         8 . The test method as claimed in    claim 5   , wherein the bio-molecule comprises nucleic acid, oligonucleotide, peptide nucleic acid, antigen, antibody, enzyme or protein.  
     
     
         9 . The test method as claimed in    claim 5   , wherein the bio-molecule binding material comprises biotin or poly-L-lysine.