US2001047522A1PendingUtilityA1
Disease resistance in vitis
Priority: Jun 26, 1996Filed: Jun 6, 2001Published: Nov 29, 2001
Est. expiryJun 26, 2016(expired)· nominal 20-yr term from priority
C12N 15/8283A01H 6/88C12N 2770/00022C07K 14/005C12N 15/8205C12N 15/8281C12N 2770/18022C12N 15/8282
52
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Claims
Abstract
Disclosed are methods for producing transgenic grape plants having resistance to bunch rot, powdery mildew, and downy mildew. Also disclosed are grape plants having resistance to bunch rot, powdery mildew, and downy mildew, wherein the plants express a Shiva lytic peptide.
Claims
exact text as granted — not AI-modified1 . A method for producing a transgenic grape cell with resistance to a fungal pathogen, said method comprising:
transforming a grape cell with a nucleic acid molecule which expresses a lytic peptide, where expression of said lytic peptide provides resistance to a fungal pathogen that causes bunch rot, powdery mildew, or downy mildew.
2 . The method of claim 1 , further comprising propagating a grape plant from said transformed grape cell.
3 . The method of claim 1 , wherein said grape cell is part of a somatic embryo.
4 . The method of claim 3 , further comprising propagating a grape plant from said somatic embryo.
5 . The method of claim 1 , wherein said transformation comprises infecting said grape cell with Agrobacterium tumefaciens comprising said nucleic acid molecule which expresses said lytic peptide.
6 . The method of claim 1 , wherein said transformation comprises bombarding said grape cell with microprojectiles comprising said nucleic acid molecule which expresses said lytic peptide.
7 . The method of claim 1 , wherein said transformation comprises bombarding said grape cell with microprojectiles, followed by infecting said bombarded cells with Agrobacterium tumefaciens comprising said nucleic acid molecule which expresses said lytic peptide.
8 . the method of claim 1 , wherein said lytic peptide is Shiva-1.
9 . The method of claim 1 , wherein said grape cell is a Vitis vinifera ‘Thompson Seedless’ grape cell.
10 . The method of claim 1 , wherein said grape cell is a scion cell.
11 . The method of claim 1 , wherein said grape cell is a rootstock cell.
12 . The method of claim 1 , wherein expression of said lytic peptide provides resistance to bunch rot.
13 . The method of claim 1 , wherein expression of said lytic peptide provides resistance to powdery mildew.
14 . The method of claim 1 , wherein expression of said lytic peptide provides resistance to downy mildew.
15 . A grape cell comprising a nucleic acid molecule which expresses a lytic peptide, wherein expression of said lytic peptide provides resistance to a fungal pathogen that causes bunch rot, powdery mildew, or downy mildew.
16 . The cell of claim 15 , wherein said nucleic acid molecule comprises an expression vector.
17 . The cell of claim 15 , wherein said cell is a scion cell.
18 . The cell of claim 15 , wherein said cell is a rootstock cell.
19 . The cell of claim 15 , wherein said cell is a somatic embryo cell.
20 . The cell of claim 15 , wherein said lytic peptide is Shiva-1.
21 . The cell of claim 15 , wherein said grape cell is a Vitis vinifera ‘Thompson Seedless’ grape cell.
22 . The cell of claim 15 , wherein expression of said lytic peptide provides resistance to bunch rot.
23 . The cell of claim 15 , wherein expression of said lytic peptide provides resistance to powdery mildew.
24 . The cell of claim 15 , wherein expression of said lytic peptide provides resistance to downy mildew.
25 . A grape plant comprising a nucleic acid molecule which expresses a lytic peptide, wherein expression of said lytic peptide provides resistance to a fungal pathogen that causes bunch rot, powdery mildew, or downy mildew.
26 . The plant of claim 25 , wherein said grape plant is a scion.
27 . The plant of claim 25 , wherein said transgenic plant is a rootstock.
28 . The plant of claim 25 , wherein said grape plant is a somatic embryo.
29 . The transgenic plant of claim 25 , wherein said grape plant is Vitis vinifera ‘Thompson Seedless.’
30 . The plant of claim 25 , wherein said nucleic acid molecule is expressed from an expression vector.
31 . The plant of claim 25 , wherein said lytic peptide is Shiva-1.
32 . The plant of claim 25 , wherein expression of said lytic peptide provides resistance to bunch rot.
33 . The plant of claim 25 , wherein expression of said lytic peptide provides resistance to powdery mildew.
34 . The plant of claim 25 , wherein expression of said lytic peptide provides resistance to downy mildew.
35 . A scion from a plant of claim 32 .
36 . A scion from a plant of claim 33 .
37 . A scion from a plant of claim 34 .
38 . A rootstock from a plant of claim 32 .
39 . A rootstock from a plant of claim 33 .
40 . A rootstock from a plant of claim 34 .
41 . A somatic embryo from a plant of claim 32 .
42 . A somatic embryo from a plant of claim 33 .
43 . A somatic embryo from a plant of claim 34 .
44 . A cell from a plant of claim 32 .
45 . A cell from a plant of claim 33 .
46 . A cell from a plant of claim 34 .
47 . A somatic embryo from a plant of claim 32 .
48 . A somatic embryo from a plant of claim 33 .
49 . A somatic embryo from a plant of claim 34 .
50 . A seed from a plant of claim 32 .
51 . A seed from a plant of claim 33 .
52 . A seed from a plant of claim 34 .Join the waitlist — get patent alerts
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