Dyeing substrates

Abstract

The invention relates to substrates, which develops colour on oxidation of the general formula 1 The invention also relates to the use of said substrates for dyeing e.g. keratinous fibers, in particular hair, dyeing textiles, a dyeing composition and a method for dyeing keratinous fibers.

1 . A substrate, which develops colour on oxidation characterized by the inclusion of an amino benzoic acid compound with the general formula 1  
       
         
           
           
               
               
           
         
       
       wherein 
 R is an amino, mono- or a distributed amino or OR′, where R is H, alkyl, alkenyl, alkynyl, halogenalkyl, nitro, benzyl, phenyl or substituted phenyl, X, Y and Z may each independently be hydrogen alkyl, alkenyl, alkynyl, halogenalkyl, nitro, benzyl, phenyl, substituted phenyl, amino, hydroxy or mercapto with the proviso that at least one of the groups X, Y and Z is an amino group or an salt thereof.  
 
     
     
         2 . The substrate according to  claim 1 , wherein R′ is a methyl, ethyl or isopropyl ester.  
     
     
         3 . The use of amino benzoic acid compounds with the general formula 1 as well as the salt thereof as a colour producing substrate in analysis based on the use of peroxidase.  
     
     
         4 . The use of amino benzoic acid compounds with the general formula 1 as well as the salt thereof as a colour producing substrate in hair dyeing compositions as well as for compositions for dying of natural and synthetic fibres including textiles, thread and yarns.  
     
     
         5 . Use according to claims  3  or  4  wherein the compound is selected from the group of methyl, ethyl or isopropyl esters of amino benzoic acid.  
     
     
         6 . Method for quantitative and/or qualitative analysis of a material of biological interest, wherein a peroxidase enzyme together with a marker is bound to the compound of interest, hydrogen peroxide is then converted with a colour producing substrate in the presence of the peroxidase, characterized by the use of a substrate selected from the group made up of amino benzoic acid compounds with the general formula 1 as well as its salts.  
     
     
         7 . The method according to  claim 8  wherein the compound is selected from the group of methyl, ethyl or isopropyl esters of amino benzoic acid as well as its salts.  
     
     
         8 . The method according to  claim 6  wherein the peroxidase marker is capable of forming an immunological link to the materiel of biological interest.  
     
     
         9 . The method according to  claim 6  wherein the materiel of biological interest is an antigen and the marker is an antibody to the antigen.  
     
     
         10 . Method for producing a colored product wherein the substrate according to  claim 1  or  2  is converted by means of an oxidation system.  
     
     
         11 . The method according to  claim 10 , wherein the oxidation system comprises an inorganic compound, preferably one of the compound hypochlorite, hypobromite, permanganate, dichromate, or iron as Fe3+).  
     
     
         12 . The method according to  claim 10 , wherein the oxidation system includes an enzyme and an electron acceptor.  
     
     
         13 . The method according to  claim 12 , wherein the enzyme is a peroxidase and the electron acceptor is hydrogen peroxide or that the enzyme is oxidase and the electron acceptor is oxygen.  
     
     
         14 . The method according to  claim 13 , wherein the oxidase is chosen from the group catechol oxidase, laccase and related enzymes o-amino phenoloxidase.  
     
     
         15 . A composition adapted for dyeing of keratinous fibres, comprising 1) at least one oxidation enzyme, 2) at least one substrate according to any of claims  1  or  2 , and optionally 3) at least one modifier.  
     
     
         16 . The composition according to  claim 15 , wherein the oxidation enzyme is an oxidoreductase selected from laccases and related enzymes, oxidases or peroxidases.  
     
     
         17 . The composition of  claim 16 , wherein the oxidation enzyme is a laccase, in particular a laccase derived from a strain of Polyporus sp., in particular a strain of  P. pinsitus  or  P. versicolor,  a strain of Myceliophthora sp., in particular  M. thermophila,  a strain of Rhizoctonia sp., in particular  Rh. praticola  or  Rh.  solani, a strain of a Rhus sp., in particular  Rhus vernicifera,  or a strain of Scytalidium, in particular  S. thermophilium,  a strain of Pyricularia sp., in particular  P. oryzae.    
     
     
         18 . A method for dyeing keratinous fibres, comprising contacting the fibers with a composition comprising 1) at least one oxidation enzyme, 2) at least one substrate according to claims  1  or  2 , and optionally 3) at least one modifier, for a period of time and under conditions sufficient to permit oxidation of the substrate to a colored compound.  
     
     
         19 . The method of  claim 18 , wherein the dyeing is carried out at a pH in the range from 3.0 to 9.0, preferably 4.0 to 8.5, especially 6.0 to 8.0.  
     
     
         20 . The method of claims  18  or  19 , wherein the composition is as defined in any of  claims 15  to  17 .