Mammalian cytokine-like receptor 5

Abstract

Novel receptor polypeptides, polynucleotides encoding the polypeptides, and related compositions. The polypeptides of the present invention can be used to down-regulate their natural ligands. The polynucleotides and subsequences thereof can be used as diagnostic probes to determine if chromosome 19 is mutated. The antibodies which bind to the polypeptides can be used to purify the receptors and to inhibit the binding of the ligands onto the receptors.

We claim:  
     
         1 . An isolated polynucleotide which encodes a mammalian polypeptide, said polypeptide being comprised of an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NOs: 17-31.  
     
     
         2 . The isolated polynucleotide of  claim 1  wherein said polynucleotide is a DNA sequence.  
     
     
         3 . The isolated polynucleotide of  claim 1  wherein said polynucleotide is an RNA sequence.  
     
     
         4 . An expression vector comprising the following operably linked elements: 
 a transcription promoter;    a DNA segment encoding a mammalian polypeptide, said polypeptide being comprised of an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NOs: 17-37; and    a transcription terminator.    
     
     
         5 . An isolated polypeptide said polypeptide being comprised of an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NOs: 17-31.  
     
     
         6 . A peptide or polypeptide which has the amino acid sequence of an epitope-bearing portion of a Zcytor5 polypeptide.  
     
     
         7 . A polypeptide of  claim 6  wherein the polypeptide has amino acid sequence of at least 15 amino acid residues.  
     
     
         8 . The polypeptide of  claim 7  wherein said polypeptide is selected from the group of polypeptide consisting of the amino acid sequences of SEQ ID NOs: 32-37.  
     
     
         9 . An antibody which specifically binds to an epitope-binding sequence of a Zcytor5 polypeptide.  
     
     
         10 . An antibody of  claim 9  wherein said antibody binds to a polypeptide comprised of an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO: 4, SEQ ID NO:6, and SEQ ID NOs: 17-37.  
     
     
         11 . An anti-idiotypic antibody of an antibody of claims  9  or  10 .  
     
     
         12 . A method for producing an antibody which binds to a Zcytor5 polypeptide comprising inoculating an animal with an epitope-bearing amino acid sequence of Zcytor5 polypeptide under conditions wherein said animal produces antibodies which bind to the Zcytor5 polypeptide; and 
 isolating said antibodies.