US2002061566A1PendingUtilityA1

Biooxidation capabilities of candida sp

49
Priority: Mar 20, 2000Filed: Mar 20, 2001Published: May 23, 2002
Est. expiryMar 20, 2020(expired)· nominal 20-yr term from priority
Inventors:L. Dudley EirichKevin W. AndersonJeffrey A. GatesC. Ron WilsonManfred BiermannGilbert H. Vice
C12P 7/02C12P 7/40
49
PatentIndex Score
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Cited by
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References
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Claims

Abstract

A bioprocess for producing carboxylic acids, alcohols and aldehydes is provided by culturing Candida sp. in a fermentation medium containing various defined substrates.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
         1 . A process for producing a carboxylic acid comprising culturing Candida sp. in a fermentation medium containing a substrate of the formula R(CH 2 ) n CH 3 wherein n is ≧1 and R is selected from the group consisting of epoxide, alkoxy, ether, saturated primary alcohol, cycloalkyl, aryl, diol and diol ester, whereby at least one terminal methyl group of the substrate is oxidized to a carboxylic acid.  
     
     
         2 . The process of  claim 1  wherein the substrate is dissolved in a solvent prior to contact with the fermentation medium.  
     
     
         3 . The process of  claim 2  wherein the solvent is an organic solvent.  
     
     
         4 . The process of  claim 3  wherein the organic solvent is selected from the group consisting of ethanol and hexane.  
     
     
         5 . The process of  claim 3  wherein the organic solvent is acetone.  
     
     
         6 . The process of claim I wherein the Candida sp. is selected from the group consisting of  C. albicans, C. cloacae, C. guillermondii, C. intermedia, C. lipolytica, C. maltosa, C. parapsilosis,  and  C. zeylenoides.    
     
     
         7 . The process of claim I wherein the Candida sp. is  C. tropicalis.    
     
     
         8 . The process of  claim 7  wherein  C tropicalis  is substantially β-oxidation pathway blocked.  
     
     
         9 . The process of  claim 8  wherein  C. tropicalis  is H5343.  
     
     
         10 . The process of  claim 8  wherein one or more P450 CYP genes, P450 CPR genes, or a combination thereof is amplified in said  C. tropicalis.    
     
     
         11 . The process of  claim 1  wherein the substrate is a compound selected from the group consisting of dodecylvinyl ether, dihexyl ether, dipentyl ether, 1-dodecanol, 2-hexyldecanol, 2-butyl-1-octanol, 1,2-hexadecanediol, epoxidized soybean oil, 1,2-epoxytetradecane, butylcyclohexane, propylcyclohexane, ethylcyclohexane, polyethylene glycol 200 monolaurate, polyethylene glycol 200 dilaurate.  
     
     
         12 . A process for producing a carboxylic acid comprising culturing Candida sp. in a fermentation medium containing a substrate selected from the group consisting of 12-hydroxystearic acid, hexadecyl pelargonate, castor oil, hexadecyl acetate, dodecene, tetradecene, hexadecene, octadecene, trans-2-nonene, 7-trans-tetradecene, 2-heptylundecanoic acid and 2-hexyldecanoic acid whereby at least one terminal methyl group of the substrate is oxidized to a carboxylic acid.  
     
     
         13 . The process of  claim 12  wherein the substrate is dissolved in a solvent prior to contact with the fermentation medium.  
     
     
         14 . The process of  claim 12  wherein the Candida sp. is selected from the group consisting of  C. albicans, C. cloacae, C. guillermondii; C. intermedia, C. lipolytica, C. maltosa, C. parapsilosis,  and  C. zeylenoides.    
     
     
         15 . The process of  claim 12  wherein the Candida sp. is  C tropicalis.    
     
     
         16 . A process for producing an alcohol comprising culturing Candida sp. in a fermentation medium containing a substrate of the formula R(CH 2 ) n CH 3 , wherein n is ≧1 and R is selected from the group consisting of epoxide, alkoxy, ether, saturated primary alcohol, cycloalkyl, aryl, diol and diol ester, whereby at least one terminal methyl group of the substrate is oxidized to an alcohol.  
     
     
         17 . The process of  claim 16  wherein the substrate is dissolved in a solvent prior to contact with the fermentation medium.  
     
     
         18 . The process of  claim 17  wherein the solvent is an organic solvent.  
     
     
         19 . The process of  claim 18  wherein the organic solvent is selected from the group consisting of ethanol and hexane.  
     
     
         20 . The process of  claim 18  wherein the organic solvent is acetone.  
     
     
         21 . The process of  claim 16  wherein the Candida sp. is selected from the group consisting of  C. albicans, C. cloacae, C. guillermondii, C. intermedia, C. lipolytica, C. maltosa, C. parapsilosis,  and  C. zeylenoides.    
     
     
         22 . The process of  claim 16  wherein the Candida sp. is  C. tropicalis.    
     
     
         23 . The process of  claim 22  wherein  C. tropicalis  is substantially β-oxidation pathway blocked.  
     
     
         24 . The process of  claim 23  wherein  C. tropicalis  is H5343.  
     
     
         25 . The process of  claim 23  wherein one or more P450 CYP genes, P450 CPR genes, or a combination thereof is amplified in said  C. tropicalis.    
     
     
         26 . The process of  claim 16  wherein the substrate is a compound selected from the group consisting of dodecylvinyl ether, dihexyl ether, dipentyl ether, 1-dodecanol, 2-hexyldecanol, 2-butyl-1-octanol, 1,2-hexadecanediol, epoxidized soybean oil, 1,2-epoxytetradecane, butylcyclohexane, propylcyclohexane, ethylcyclohexane, polyethylene glycol 200 monolaurate, polyethylene glycol 200 dilaurate.  
     
     
         27 . A process for producing an alcohol comprising culturing Candida sp. in a fermentation medium containing a substrate selected from the group consisting of 12-hydroxystearic acid, hexadecyl pelargonate, castor oil, hexadecyl acetate, dodecene, tetradecene, hexadecene, octadecene, trans-2-nonene, 7-trans-tetradecene, 2-heptylundecanloic acid and 2-hexyldecanoic acid, whereby at least one terminal methyl group of the substrate is oxidized to an alcohol.  
     
     
         28 . The process of  claim 27  wherein the substrate is dissolved in a solvent prior to contact with the fermentation medium.  
     
     
         29 . The process of  claim 27  wherein the Candida sp. is selected from the group consisting of  C. albicans, C. cloacae, C. guillermondii, C. intermedia, C. lipolytica, C. maltosa, C. parapsilosis,  and  C. zeylenoides.    
     
     
         30 . The process of  claim 27  wherein the Candida sp. is  C. tropicalis .

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