Molecular sequence of swine retrovirus and methods of use

Abstract

This invention relates to newly identified polynucleotides, polypeptides encoded by such polynucleotides, the use of such polynucleotides and polypeptides, as well as the production of such polynucleotides and polypeptides. More particularly, the polypeptides of the present invention are PERV-D env polypeptides. The invention also relates to detecting the presence of a porcine retrovirus in tissue comprising such polypeptides or polynucleotides that encode the polypeptides.

What is claimed is:  
     
         1 . An isolated polynucleotide, comprising: 
 a polynucleotide having at least 95% identity to a member selected from the group consisting of: 
 (a) a polynucleotide encoding a PERV-D polypeptide comprising amino acids 1 to 91 of SEQ ID NO: 2; and  
 (b) the complement of (a).  
   
     
     
         2 . The isolated polynucleotide of  claim 1 , wherein said member is (a).  
     
     
         3 . A method of making a recombinant vector comprising inserting the isolated polynucleotide of  claim 2  into a vector wherein the polynucleotide is DNA.  
     
     
         4 . A recombinant vector comprising the isolated polynucleotide of  claim 2 , wherein the polynucleotide is DNA.  
     
     
         5 . A recombinant host cell comprising the isolated polynucleotide of  claim 2  wherein said polynucleotide is DNA.  
     
     
         6 . The isolated polynucleotide of  claim 2  comprising a polynucleotide encoding a polypeptide consisting of the amino acids 1 to 91 of SEQ ID NO: 2.  
     
     
         7 . A method for producing a polypeptide comprising expressing from the recombinant cell of  claim 5  the polypeptide encoded by said polynucleotide.  
     
     
         8 . An isolated polypeptide comprising an amino acid sequence encoded by the isolated polynucleotide of  claim 2 .  
     
     
         9 . The isolated polypeptide of  claim 8  comprising amino acids 1 to 91 of SEQ ID NO:2.  
     
     
         10 . A method for detecting the presence of a porcine retrovirus in a tissue sample comprising detecting the presence of a polypeptide of  claim 8 .  
     
     
         11 . An isolated polynucleotide, comprising: 
 a polynucleotide having at least 95% identity to a member selected from the group consisting of: 
 (a) a polynucleotide comprising the sequence shown as nucleotides 1-275 of SEQ ID NO: 1; and  
 (b) the complement of (a).  
   
     
     
         12 . The isolated polynucleotide of  claim 11 , wherein said member is (a).  
     
     
         13 . A method of making a recombinant vector comprising inserting the isolated polynucleotide of  claim 12  into a vector, wherein said polynucleotide is DNA.  
     
     
         14 . A recombinant vector comprising the isolated polynucleotide of  claim 12  wherein said polynucleotide is DNA.  
     
     
         15 . A recombinant host cell comprising the isolated polynucleotide of  claim 12  wherein said polynucleotide is DNA.  
     
     
         16 . The isolated polynucleotide of  claim 12  comprising a polynucleotide consisting of the nucleotides 1-275 of SEQ ID NO: 1.  
     
     
         17 . A method for producing a polypeptide, comprising expressing from the recombinant cell of  claim 15  the polypeptide encoded by said isolated polynucleotide.  
     
     
         18 . An isolated polypeptide comprising an amino acid sequence encoded by the polynucleotide of  claim 12 .  
     
     
         19 . A method for detecting the presence of a porcine retrovirus in a tissue sample comprising detecting the presence of a polypeptide of  claim 18 .  
     
     
         20 . An isolated polynucleotide, comprising at least 30 consecutive bases of a polynucleotide having the sequence shown as nucleotides 1-275 of SEQ ID NO: 1.  
     
     
         21 . An isolated polynucleotide of  claim 20 , comprising at least 50 consecutive bases of a polynucleotide having the sequence shown as nucleotides 1-275 of SEQ ID NO:1.  
     
     
         22 . An isolated polynucleotide of  claim 20 , comprising at least 75 consecutive bases of a polynucleotide having the sequence shown as nucleotides 1-275 of SEQ ID NO: 1.  
     
     
         23 . A method of making a recombinant vector comprising inserting the isolated polynucleotide of  claim 20  into a vector.  
     
     
         24 . A recombinant vector comprising the isolated polynucleotide of  claim 20  wherein said polynucleotide is DNA.  
     
     
         25 . A recombinant vector comprising the isolated polynucleotide of  claim 21  wherein said polynucleotide is DNA.  
     
     
         26 . A recombinant host cell comprising the isolated polynucleotide of  claim 20  wherein said polynucleotide is DNA.  
     
     
         27 . A recombinant host cell comprising the isolated polynucleotide of  claim 22  wherein said polynucleotide is DNA.  
     
     
         28 . A method for producing a polypeptide, comprising expressing from the recombinant cell of  claim 26  the polypeptide encoded by said polynucleotide.  
     
     
         29 . A method for producing a polypeptide, comprising expressing from the recombinant cell of  claim 27  the polypeptide encoded by said polynucleotide.  
     
     
         30 . An isolated polypeptide comprising an amino acid sequence encoded by a polynucleotide of  claim 20 .  
     
     
         31 . An isolated polypeptide comprising an amino acid sequence encoded by a polynucleotide of  claim 21 .  
     
     
         32 . An isolated polypeptide, comprising an amino acid sequence encoded by a polynucleotide which is at least 95% identical to the isolated polynucleotide of  claim 20 .  
     
     
         33 . An isolated polypeptide, comprising an amino acid sequence encoded by a polynucleotide which is at least 95% identical to the isolated polynucleotide of  claim 21 .  
     
     
         34 . An isolated polypeptide, comprising an amino acid sequence encoded by a polynucleotide which is at least 95% identical to the isolated polynucleotide of  claim 22 .  
     
     
         35 . An antibody against the polypeptide of  claim 32 .  
     
     
         36 . An antibody against the polypeptide of  claim 22 .  
     
     
         37 . A method for detecting the presence of a porcine retrovirus in a tissue sample comprising detecting the presence of a polypeptide of  claim 32 .  
     
     
         38 . A method for creating passive immunity in a host comprising administering an effective amount of an antibody according to  claim 35 .  
     
     
         39 . A method for creating passive immunity in a host comprising administering an effective amount of an antibody according to  claim 36 .  
     
     
         40 . A vaccine for immunizing a host against a porcine retrovirus, comprising: 
 (a) administering an immunizing effective amount of a polypeptide of claim  8 ; or    (b) administering an isolated polynucleotide of  claim 2 , such that its encoded polypeptide is expressed in an immunizing effect amount.    
     
     
         41 . A vaccine for immunizing a host against a porcine retrovirus, comprising: 
 (a) administering an immunizing effective amount of a polypeptide of claim  18 ; or    (b) administering an isolated polynucleotide of  claim 12 , such that its encoded polypeptide is expressed in an immunizing effect amount.    
     
     
         42 . A vaccine for immunizing a host against a porcine retrovirus, comprising: 
 (a) administering an immunizing effective amount of a polypeptide of claim  30 ; or    (b) administering an isolated polynucleotide of  claim 20 , such that its encoded polypeptide is expressed in an immunizing effect amount.