US2002072094A1PendingUtilityA1

Method of producing particles containing nucleic acids sequences in yeast

20
Priority: Jul 12, 1996Filed: Jul 29, 1998Published: Jun 13, 2002
Est. expiryJul 12, 2016(expired)· nominal 20-yr term from priority
Inventors:B. Duane PriceRoland RueckertMichael KrolPaul G. Ahlquist
C12N 2770/16022C12N 2770/30022C12N 15/81C07K 14/005
20
PatentIndex Score
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Cited by
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References
0
Claims

Abstract

A method of synthesizing particles containing nucleic acid sequences is disclosed. This method comprises the steps of introducing a non-endogenous packageable nucleic acid sequence and a sequence encoding a suitable non-endogenous capsid protein into yeast cells and inducing particle assembly, wherein at least one nucleic acid is encapsidated. Preferably, the particle is an infectious virion and the method additionally comprises the step of inducing viral nucleic acid replication before particle assembly. A method of replicating an animal or Nodaviridae virus nucleic acid sequences in yeast is also disclosed.

Claims

exact text as granted — not AI-modified
We claim:  
     
         1 . A method of synthesizing particles containing nucleic acid sequences comprising the steps of 
 a) introducing a non-endogenous packageable nucleic acid sequence and a nucleic acid sequence encoding non-endogenous suitable capsid proteins into yeast cells, and    b) inducing particle assembly, wherein at least one nucleic acid molecule is encapsidated within the particle.    
     
     
         2 . The method of  claim 1  wherein the particle is an infectious virion.  
     
     
         3 . The method of  claim 1  additionally comprising the steps of introducing a replicable nucleic acid sequence and a sequence encoding suitable nucleic acid replication proteins into the yeast cells and inducing viral nucleic acid replication before particle assembly.  
     
     
         4 . The method of  claim 1  wherein the packageable nucleic acid sequence or the sequence encoding non-endogenous suitable capsid proteins comprises animal or plant virus sequences.  
     
     
         5 . The method of  claim 1  wherein the packageable nucleic acid sequence or the sequence encoding non-endogenous suitable capsid proteins comprises RNA virus sequences.  
     
     
         6 . The method of  claim 5  wherein the packageable nucleic acid sequence or the sequence encoding non-endogenous suitable capsid proteins comprises (+) strand RNA virus sequences.  
     
     
         7 . The method of  claim 6  wherein the packageable nucleic acid sequence or the sequence encoding non-endogenous suitable capsid proteins comprises sequences from a member of the Nodaviridae or Bromoviridae family.  
     
     
         8 . The method of  claim 7  wherein the packageable nucleic acid sequence or the sequence encoding non-endogenous suitable capsid proteins comprises flock house virus or brome mosaic virus sequences.  
     
     
         9 . The method of  claim 1  wherein the packageable nucleic acid sequence or the sequence encoding non-endogenous suitable capsid proteins comprises sequences from more than one virus.  
     
     
         10 . The method of  claim 1  wherein the packageable nucleic acid sequence or the sequence encoding non-endogenous suitable capsid proteins comprises non-viral sequences.  
     
     
         11 . The method of  claim 1  wherein the packageable nucleic acid sequence or the sequence encoding non-endogenous suitable capsid proteins comprises synthetically created sequences.  
     
     
         12 . The method of  claim 1  wherein the yeast is of or associated with the family Saccharomycetaceae.  
     
     
         13 . The method of  claim 1  wherein at least a portion of the introduced sequences is transfected into the yeast.  
     
     
         14 . The method of  claim 1  wherein at least a portion of the introduced sequences is located on the yeast chromosome.  
     
     
         15 . The method of  claim 1  wherein at least a portion of the introduced sequences is located on a nucleic acid replicon.  
     
     
         16 . A product of the method of  claim 1 .  
     
     
         17 . A product of the method of  claim 2 .  
     
     
         18 . A product of the method of  claim 3 .  
     
     
         19 . A product of the method of  claim 9 .  
     
     
         20 . A product of the method of  claim 10 .  
     
     
         21 . A product of the method of  claim 11 .  
     
     
         22 . A method of replicating an animal virus nucleic acid sequence, comprising the steps of 
 a) introducing a replicable nucleic acid sequence and sequence encoding a suitable nucleic acid replication protein into yeast cells, wherein the replicable nucleic acid sequence or the sequence encoding a suitable nucleic acid replication protein comprises an animal virus sequence, and    b) inducing viral nucleic acid replication.    
     
     
         23 . The method of  claim 22  wherein the yeast is of or associated with the family Saccharomycetaceae.  
     
     
         24 . The product of the method of  claim 22 .  
     
     
         25 . A method of replicating a Nodaviridae virus nucleic acid sequence, comprising the steps of 
 a) introducing a replicable nucleic acid sequence and a sequence encoding a suitable nucleic acid replication protein into yeast cells, wherein the replicable nucleic acid sequence or the sequence encoding a suitable nucleic acid replication protein comprises a Nodavirus sequence, and    b) inducing viral nucleic acid replication.    
     
     
         26 . The method of  claim 25  wherein replicable nucleic acid sequence or the sequence encoding a suitable nucleic acid replication protein comprises flock house virus sequences.  
     
     
         27 . The method of  claim 25  wherein the yeast is of or associated with the family Saccharomycetaceae.  
     
     
         28 . The product of the method of  claim 25 .  
     
     
         29 . The method of  claim 22  wherein the replicable nucleic acid sequence or the sequence encoding a suitable nucleic acid replication protein comprises a non-viral sequence.  
     
     
         30 . The method of  claim 29  wherein the non-viral sequence is a protein coding sequence.  
     
     
         31 . The method of  claim 30  additionally comprising the step of expressing the protein coding sequence.  
     
     
         32 . The replication product of the method of  claim 29 .  
     
     
         33 . The expression product of the method of  claim 31 .  
     
     
         34 . The method of  claim 25  wherein the replicable nucleic acid sequence or the sequence encoding a suitable nucleic acid replication protein comprises a non-viral sequence.  
     
     
         35 . The method of  claim 34  wherein the non-viral sequence is a protein coding sequence.  
     
     
         36 . The method of  claim 35  additionally comprising the step of expressing the protein coding sequence.  
     
     
         37 . The replication product of the method of  claim 34 .  
     
     
         38 . The expression product of the method of claim  36 .

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