US2002086367A1PendingUtilityA1

Novel secreted proteins

Priority: Jun 30, 2000Filed: Jun 29, 2001Published: Jul 4, 2002
Est. expiryJun 30, 2020(expired)· nominal 20-yr term from priority
C07K 2319/00C07K 14/52C07K 14/47
59
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Claims

Abstract

The present invention provides to polynucleotides and secreted proteins encoded by the polynucleotides. The proteins include and cytokines, growth factors, include fusion proteins operably linked to a second polypeptide. The invention further provides therapeutic and diagnostic methods utilizing the polynucleotides, polypeptides, and antagonists of the polypeptides.

Claims

exact text as granted — not AI-modified
We claim:  
     
         1 . An isolated polypeptide comprising residues 23 to 150 as shown in SEQ ID NO:2.  
     
     
         2 . The isolated polypeptide of  claim 1  wherein the polypeptide further comprises residues 1 to 150 as shown in SEQ ID NO:2.  
     
     
         3 . An isolated polynucleotide comprising a sequence of nucleotides, wherein the sequence encodes the isolated polypeptide of  claim 1 .  
     
     
         4 . An expression vector comprising the following operably linked elements: 
 a transcription promoter;    a DNA segment having the isolated polynucleotide of claim  3 ; and    a transcription terminator.    
     
     
         5 . A cultured cell comprising the expression vector of  claim 4 .  
     
     
         6 . A method of producing a polypeptide comprising culturing the cell of  claim 5  under conditions whereby said sequence of nucleotides is expressed, and recovering said polypeptide.  
     
     
         7 . A polypeptide produced by the method of  claim 6 .  
     
     
         8 . An isolated polynucleotide comprising the polynucleotide sequence as shown in SEQ ID NO:1.  
     
     
         9 . An antibody that specifically binds to the isolated protein of  claim 1 .  
     
     
         10 . An isolated polypeptide comprising residues 19 to 117 as shown in SEQ ID NO:5.  
     
     
         11 . The isolated polypeptide of  claim 10  wherein the polypeptide further comprises residues 1 to 117 as shown in SEQ ID NO:5.  
     
     
         12 . An isolated polynucleotide comprising a sequence of nucleotides, wherein the sequence encodes the isolated polypeptide of  claim 10 .  
     
     
         13 . An expression vector comprising the following operably linked elements: 
 a transcription promoter;    a DNA segment having the isolated polynucleotide of claim  12 ; and    a transcription terminator.    
     
     
         14 . A cultured cell comprising the expression vector of  claim 13 .  
     
     
         15 . A method of producing a polypeptide comprising culturing the cell of  claim 14  under conditions whereby said sequence of nucleotides is expressed, and recovering said polypeptide.  
     
     
         16 . A polypeptide produced by the method of  claim 15   
     
     
         17 . An isolated polynucleotide comprising the polynucleotide sequence as shown in SEQ ID NO:4.  
     
     
         18 . An antibody that specifically binds to the isolated protein of  claim 10 .  
     
     
         19 . An isolated polypeptide comprising residues 20 to 185 as shown in SEQ ID NO:8.  
     
     
         20 . The isolated polypeptide of  claim 19  wherein the polypeptide further comprises residues 1 to 185 as shown in SEQ ID NO:8.  
     
     
         21 . An isolated polynucleotide comprising a sequence of nucleotides, wherein the sequence encodes the isolated polypeptide of  claim 19 .  
     
     
         22 . An expression vector comprising the following operably linked elements: 
 a transcription promoter;    a DNA segment having the isolated polynucleotide of claim  21 ; and    a transcription terminator.    
     
     
         23 . A cultured cell comprising the expression vector of  claim 22 .  
     
     
         24 . A method of producing a polypeptide comprising culturing the cell of  claim 23  under conditions whereby said sequence of nucleotides is expressed, and recovering said polypeptide.  
     
     
         25 . A polypeptide produced by the method of  claim 24 .  
     
     
         26 . An isolated polynucleotide comprising the polynucleotide sequence as shown in SEQ ID NO:7.  
     
     
         27 . An antibody that specifically binds to the isolated protein of claim  19 .

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