US2002090611A1PendingUtilityA1

Detection of human immunodeficiency virus

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Assignee: UNIV MASSACHUSETTS A MASSACHUSPriority: Jan 8, 1999Filed: Jan 10, 2002Published: Jul 11, 2002
Est. expiryJan 8, 2019(expired)· nominal 20-yr term from priority
Inventors:Mario Stevenson
C12Q 1/703
50
PatentIndex Score
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Claims

Abstract

The invention relates to a method of detecting a HIV-infected cell in a mammal. The method includes detecting an HIV 2-LTR circle DNA molecule obtained from a cell of an HIV-positive mammal, especially an HIV-1-positive human.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
         1 . A method of detecting an HIV-infected cell from a mammal undergoing combination anti-HIV drug therapy, the method comprising detecting an HIV 2-LTR circle DNA molecule obtained from a cell of the mammal, wherein the presence of a 2-LTR circle DNA indicates an HIV-infected cell.  
     
     
         2 . The method of  claim 1 , further comprising amplifying the DNA molecule before the detecting step.  
     
     
         3 . The method of  claim 2 , wherein the DNA molecule is amplified using polymerase chain reaction.  
     
     
         4 . The method of  claim 1 , wherein the drug therapy comprises administering to the mammal at least one HIV reverse transcriptase inhibitor.  
     
     
         5 . The method of  claim 4 , wherein the drug therapy further comprises administering to the mammal at least one HIV protease inhibitor.  
     
     
         6 . The method of  claim 1 , wherein the drug therapy comprises administering to the mammal at least one HIV protease inhibitor.  
     
     
         7 . The method of  claim 1 , wherein the mammal is an HIV-1-positive mammal.  
     
     
         8 . The method of  claim 7 , wherein the mammal is a human.  
     
     
         9 . The method of  claim 1 , wherein the mammal is a human.  
     
     
         10 . The method of  claim 1 , wherein the cell is a peripheral blood mononuclear cell.  
     
     
         11 . The method of  claim 1 , wherein cell-free HIV viral RNA cannot be detected in the blood of the mammal.  
     
     
         12 . A method of detecting an HIV-infected cell in a mammal, the method comprising detecting an HIV 2-LTR circle DNA molecule obtained from a cell of a mammal, wherein cell-free HIV viral RNA cannot be detected in the blood of the mammal, and wherein the presence of a 2-LTR circle DNA indicates a HIV-infected cell.  
     
     
         13 . The method of  claim 12 , further comprising amplifying the DNA molecule before the detecting step.  
     
     
         14 . The method of  claim 13 , wherein the DNA molecule is amplified using polymerase chain reaction.  
     
     
         15 . The method of  claim 12 , wherein the mammal is an HIV-1-positive mammal.  
     
     
         16 . The method of  claim 15 , wherein the mammal is a human.  
     
     
         17 . The method of  claim 12 , wherein the mammal is a human.  
     
     
         18 . The method of  claim 12 , wherein the cell is a peripheral blood mononuclear cell.  
     
     
         19 . A method of detecting an HIV-1-infected peripheral blood mononuclear cell (PBMC) in an individual, the method comprising 
 amplifying an HIV-1 2-LTR circle DNA molecule obtained from a PBMC of an HIV-1-positive individual undergoing combination anti-HIV-1 drug therapy, to produce an amplified nucleic acid, wherein cell-free HIV-1 viral RNA cannot be detected in the blood of the individual; and    detecting the amplified nucleic acid, wherein the presence of the amplified nucleic acid indicates the presence an HIV-infected PBMC.    
     
     
         20 . A method of  claim 1 , further comprising obtaining the HIV 2-LTR circle DNA molecule using an alkaline lysis method.  
     
     
         21 . A method of  claim 3 , wherein the primers used for PCR comprise a (−) strand primer spanning nucleotides 9591 to 9610 of the HXB2 strain of HIV-1, and a (+) strand primer spanning nucleotides 9650-9669 of the HXB2 strain of HIV-1.  
     
     
         22 . A method of treatment for HIV infection in a mammal, the method comprising 
 administering to the mammal one or more anti-HIV agents in an amount effective to reduce an HIV viral load in the mammal; and    detecting HIV-infected cells in the mammal using the method of  claim 1 ,    wherein treatment is continued until the level of HIV-infected cells falls below 1 in one million peripheral blood mononuclear cell.

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