US2002096273A1PendingUtilityA1

Fungi for improvements of wood and pulp appearance and qualities

Priority: Jun 7, 2000Filed: Mar 30, 2001Published: Jul 25, 2002
Est. expiryJun 7, 2020(expired)· nominal 20-yr term from priority
C12R 2001/645B27K 5/00C12N 1/145B27K 2240/20B27K 3/002A01H 15/00
38
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Claims

Abstract

The invention relates to the use of fungi stains of Ophiostoma floccosum, Ophiostoma piceae or Ophiostoma pluruanulatum , or mixtures on wood and pulp to improve chemical pulping processes and/or reduce cooking tie and/or improve brightness and/or decrease extractives.

Claims

exact text as granted — not AI-modified
1 . An inoculum for wood comprising or including one or more forms of a fungal culture of the species  Ophiostoma floccosum  and/or  Ophiostoma piceae  and/or  Ophiosotoma pluruanulatum  effective to reduce the amount of colour staining caused by wood staining fungi.  
     
     
         2 . An inoculum for wood as claimed in  claim 1  wherein the fungal culture forms are biologically pure.  
     
     
         3 . An inoculum for wood as claimed in  claim 1  wherein the fungal culture is selected from the following strains: 
   Ophiosotoma floccosum  F71,  
   Ophiosotoma floccosum  F80,  
   Ophiosotoma floccosum  F93,  
   Ophiosotoma floccosum  F40,  
   Ophiosotoma floccosum  F13,  
   Ophiostoma piceae  OPC 703,  
   Ophiostoma piceae  OPC 422,  
   Ophiostoma piceae  OPC 580,  
   Ophiostoma piceae  OPC 194,  
   Ophiostoma pluruanulatum  3410,  
   Ophiostoma pluruanulatum  7073,  
   Ophiostoma pluruanulatum  5040,  
   Ophiostoma pluruanulatum  4650  
 
     
     
         4 . An inoculum for wood as claimed in  claim 1  wherein the wood source may be wood chips and the fungus or fungi may be applied by spraying the wood chips.  
     
     
         5 . An inoculum for wood as claimed in  claim 1  wherein the wood source may be logs to be cut into structural wood, and inoculation includes inoculating at least one end of the logs, and preferably all around the logs' surfaces.  
     
     
         6 . An inoculum for wood as claimed in  claim 1  therein the wood source may be structural wood and inoculation includes inoculating at least 60% of the surface area of the structural wood.  
     
     
         7 . An inoculum for wood as claimed in  claim 1  wherein the wood source may be structural wood and inoculation includes inoculating at least 60% of the surface area of the structural wood, which may then be later made into chips or a subsequent fibre product.  
     
     
         8 . An inoculum for wood as claimed in  claim 1  wherein the wood to be inoculated may be a conifer such as but not limited to Radiata pine, Douglas fir or Loblolly pine, and it may also be a hardwood including but not limited to eucalyptus, oak, poplar, or aspen.  
     
     
         9 . A biologically pure culture of a strain of  Ophiostoma floccosum  having all of the identifying characteristics of one of the fungi of stains F40, F13, F71, F80 and F93.  
     
     
         10 . A biologically pure culture of a strain of  Ophiostoma piceae  having all of the identifying characteristics of one of the fungi of strains OPC 703, OPC 422, OPC 580 and OPC 194.  
     
     
         11 . A biologically pure culture of a strain of  Ophiostoma pluruanulatum  having all of the identifying characteristics of one of the fungi of strains 3410, 7073, 5040 and 4650.  
     
     
         12 . A method of reducing the amount of colour staining caused by wood staining fungi in and/or on a wood source which comprises or includes inoculating at least a portion of the wood source with an effective amount of at least one fungus selected from the group consisting of the species  Ophiosotoma floccosum, Ophiosotoma piceae  and  Ophiostoma pluruanulatum , which is capable of reducing the amount of colour staining caused by the wood staining fungi.  
     
     
         13 . A method of reducing the amount of colour staining as claimed in  claim 12  wherein the fungi may be selected from the following stains: 
   Ophiosotoma floccosum  F13,  
   Ophiosotoma floccosum  F40,  
   Ophiosotoma floccosum  F71,  
   Ophiosotoma floccosum  F80,  
   Ophiosotoma floccosum  F93,  
   Ophiostoma piceae  OPC 703,  
   Ophiostoma piceae  OPC 422,  
   Ophiostoma piceae  OPC 580,  
   Ophiostoma piceae  OPC 194,  
   Ophiostoma pluruanulatum  3410,  
   Ophiostoma pluruanulatum  7073,  
   Ophiostoma pluruanulatum  5040,  
   Ophiostoma pluruanulatum  4650  
 
     
     
         14 . A method of reducing the amount of colour staining as claimed in  claim 12  wherein the wood source may be wood chips and the fungus or fungi may be applied by spraying the wood chips.  
     
     
         15 . A method of reducing the amount of colour staining as claimed in  claim 12  wherein the wood source may be logs to be cut into structural wood, and inoculation includes inoculating at least one end of the logs, and preferably all around the logs' surfaces.  
     
     
         16 . A method of reducing the amount of colour staining as claimed in  claim 12  wherein the wood source may be structural wood and inoculation includes inoculating at least 60% of the surface area of the structural wood.  
     
     
         17 . A method of reducing the amount of colour staining as claimed in  claim 12  wherein the wood source may derive from a conifer such as but not limited to, Radiata pine, Douglas fir or Loblolly pine and it may also be a hardwood including but not limited to eucalyptus, oak, poplar, or aspen.  
     
     
         18 . An inoculum for wood comprising or including one or more forms of a fungal culture of the species  Ophiostoma floccosum,  and/or  Ophiostoma piceae  and/or  Ophiosotoma pluruanulatum  effective in reducing the pitch content of wood.  
     
     
         19 . An inoculum for wood as claimed in  claim 18  wherein the fungal culture forms are biologically pure.  
     
     
         20 . An inoculum for wood as claimed in  claim 18  wherein the fungi may be selected from the following strains: 
   Ophiosotoma floccosum  F40  
   Ophiosotoma floccosum  F13  
   Ophiosotoma piceae  OPC 422  
   Ophiosotoma piceae  OPC 580  
   Ophiosotoma piceae  OPC 194  
 
     
     
         21 . An inoculum for wood as claimed in  claim 18  wherein the wood source may be wood chips and the fungus or fungi may be applied by spraying the wood chips.  
     
     
         22 . An inoculum for wood as claimed in  claim 18  wherein the wood source may be logs to be cut into structural wood, and inoculation includes inoculating at least one end of the logs.  
     
     
         23 . An inoculum for wood as claimed in  claim 18  wherein the wood source may be structural wood and inoculation includes inoculating at least 60% of the surface area of the structural wood.  
     
     
         24 . An inoculum for wood as claimed in  claim 18  wherein wood to be inoculated may be but is not limited to a conifer such as Radiata pine, Douglas fir or Loblolly pine and it may also be a hardwood including but not limited to eucalyptus, oak, poplar, or aspen.  
     
     
         25 . A biologically pure culture of a strain of  Ophiostoma floccosum  having all of the identifying characteristics of one of the fungi of stains F40 and F13.  
     
     
         26 . A biologically pure culture of a strain of  Ophiostoma piceae  having all of the identifying characteristics of one of the fungi of strain OPC 422, OPC 580, OPC 194.  
     
     
         27 . A method for reducing the pitch content in a wood source, and/or improving brightness in chemical pulp resulting from the albino treatment of wood, and/or improving pulping efficiency such that lower kappa numbers were achieved with treatment of the wood with the albino strains, which comprises or includes inoculating at least a portion of the wood source with an effective amount of at least one fungus selected from the group consisting of the species  Ophiosotoma floccosum, Ophiosotoma piceae  and  Ophiostoma pluruanulatum , which is capable of reducing the pitch content.  
     
     
         28 . A method as claimed in  claim 27  wherein the fungi may be selected from the following strains: 
   Ophiosotoma floccosum  F40  
   Ophiosotoma floccosum  F13  
   Ophiostoma piceae  OPC 422  
   Ophiostoma piceae  OPC 580  
   Ophiostoma piceae  OPC 194  
 
     
     
         29 . A milked as claimed in  claim 27  wherein the method further includes the step of maintaining the inoculated wood source under conditions which allow fungal growth from the inoculation for a term sufficient to effect a reduction of the pitch content of the wood source by such inoculated fungal growth.  
     
     
         30 . A method as claimed in  claim 27  wherein the wood source may be pulpwood, or unsterilised pulpwood, or unsterilised refined pulpwood.  
     
     
         31 . A method as claimed in  claim 27  wherein the wood source may be wood chips and the fungus or fungi is applied by spraying the wood chips.  
     
     
         32 . A method as claimed in  claim 27  wherein the wood source may be debarked or undebarked timber or logs.  
     
     
         33 . A method as claimed in  claim 27  wherein the wood source may derive from but not limited to a conifer such as Radiata pine, Douglas fir or Loblolly pine and it may also be a hardwood including but not limited to eucalyptus, oak, poplar, or aspen.  
     
     
         34 . A method as claimed in  claim 27  wherein the method may include using or applying more than one fungal strain.  
     
     
         35 . A biologically pure culture of a strain of  Ophiostoma floccosum  having all of the identifying characteristics of one of the fungi of AGAL Accession Numbers NM00/12246, NM00/12247, NM00/12490, NM00/12491 or NM00/12492.  
     
     
         36 . A biologically pure culture of a stain of  Ophiostoma piceae  having all of the identifying characteristics of one of the fungi of AGAL Accession Numbers NM00/12248, NM00/12249, NM00/12250 or NM00/12489.  
     
     
         37 . A biologically pure culture of a strain of  Ophiostoma pluruanulatum  having all of the identifying characteristics of one of the fungi of AGAL Accession Numbers NM00/12251, NM00/12252,NM00/12253 or NM00/12493.  
       
         
           
                 
               
                   TABLE 11 
                 
                     
                 
                     
                 
                   Extractive Analysis of Lab and Mill Research with  O. floccosum  strains OF40 and OF13 
                 
                     
                 
                     
                 
                 
                 
                 
                 
                 
               
                     
                     
                   % Fatty acids 
                   % Resin acids 
                     
                 
                     
                   Total 
                   on OD 
                   on 
                   % Triglicerides on 
                 
                   Treatment 
                   % MTBE 
                   Wood 
                   OD wood 
                   OD Wood 
                 
                     
                 
                   Frozen control 
                   1.97 
                   0.14 
                   0.67 
                   0.16 
                 
                   F13 1/10 YM 
                   0.71 
                   0.08 
                   0.13 
                   0.03 
                 
                   F13 × YM 
                   1.09 
                   0.08 
                   0.31 
                   0.02 
                 
                   F13 10 × YM 
                   1.32 
                   0.09 
                   0.42 
                   0.01 
                 
                   F40 ′1/10 × YM 
                   1.88 
                   0.12 
                   0.77 
                   0.1 
                 
                   F40 × YM 
                   1.14 
                   0.09 
                   0.24 
                   0.03 
                 
                   F40 10 × YM 
                   1 
                   0.02 
                   0.41 
                   0.02 
                 
                   F13 + F40 × YM 
                   1.75 
                   0.14 
                   0.65 
                   0.09 
                 
                   F13 1/10 × Peptone 
                   1.15 
                   0.03 
                   0.5 
                   0.03 
                 
                   F13 × peptone 
                   0.59 
                   0.02 
                   0.14 
                   0.01 
                 
                   F13 10 × peptone 
                   0.58 
                   0.03 
                   0.18 
                   0.01 
                 
                   F40 1/10 × peptone 
                   1.04 
                   0.07 
                   0.37 
                   0.07 
                 
                   F40 × Peptone 
                   0.9 
                   0.03 
                   0.28 
                   0.01 
                 
                   F40 10 × peptone 
                   1.28 
                   0.04 
                   0.5 
                   0.02 
                 
                   F13 1/10 × powder 
                   1.04 
                   0.08 
                   0.35 
                   0.03 
                 
                   F13 × powder 
                   0.56 
                   0.06 
                   0.16 
                   0.03 
                 
                   F13 10 × powder 
                   1.59 
                   0.1 
                   0.47 
                   0.04 
                 
                   F40 1/10 × powder 
                   1.15 
                   0.08 
                   0.29 
                   0.04 
                 
                   F40 × powder 
                   0.91 
                   0.08 
                   0.20 
                   0.03 
                 
                   F40 10 × powder 
                   0.97 
                   0.1 
                   0.19 
                   0.05 
                 
                   Mix F13 + F40 1/10 
                   0.74 
                   0.07 
                   0.14 
                   0.03 
                 
                   powder 
                 
                   Mix F13 + F40 × 
                   1.06 
                   0.11 
                   0.27 
                   0.04 
                 
                   powder 
                 
                   Mix F13 + F40 10 × 
                   1 
                   0.11 
                   0.24 
                   0.04 
                 
                   powder 
                 
                     
                 
                 
               
                   Mill Trial 
                 
                 
                 
                 
                 
                 
               
                     
                   % MTBE 
                     
                     
                   % 
                 
                     
                   extractives 
                   % Fatty acids 
                   % Resin acids 
                   Triglycerides  
                 
                   Treatment 
                   On OD wood 
                   on OD wood 
                   on OD wood 
                   on OD wood 
                 
                     
                 
                   F40 0 Wk 
                   1.94 
                   0.09 
                   0.94 
                   0.31 
                 
                   F40 2 wk 
                   2.39 
                   0.15 
                   1.23 
                   0.21 
                 
                   F40 4 wk 
                   1.47 
                   0.08 
                   0.67 
                   0.04 
                 
                   F40 6 WK 
                   1.86 
                   0.06 
                   0.93 
                   0.04 
                 
                   F40 8 wk 
                   1.41 
                   0.04 
                   0.63 
                   0.03 
                 
                   F40 10 wk 
                   1.16 
                   0.04 
                   0.46 
                   0.01 
                 
                   F40 pulp 
                   0.92 
                   0.05 
                   0.37 
                   0.02 
                 
                   Chemiwasher 
                 
                   F40 pulp Caustic 
                   0.23 
                   0.02 
                   0.06 
                   0.02 
                 
                   F40 pulp TWWP 
                   0.1* 
                   0.0 
                   0.01 
                   0.01 
                 
                   F40 pulp bleached 
                   0.1* 
                   0.01 
                   0.01 
                   0.01 
                 
                   F13 + F40 2 wk 
                   1.71 
                   0.1 
                   0.72 
                   0.18 
                 
                   F13 + F40 4 wk 
                   1.45 
                   0.09 
                   0.55 
                   0.08 
                 
                   F13 + F40 6 wk 
                   2.24 
                   0.09 
                   1.09 
                   0.06 
                 
                   F13 + F40 8 Wk 
                   1.15 
                   0.05 
                   0.38 
                   0.03 
                 
                   F13 + F40 10 wk 
                   1.4 
                   0.07 
                   0.49 
                   0.02.

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