US2002147148A1PendingUtilityA1

Insecticidal protein toxins from xenorhabdus

55
Priority: May 5, 1997Filed: Feb 19, 2002Published: Oct 10, 2002
Est. expiryMay 5, 2017(expired)· nominal 20-yr term from priority
Inventors:Jerald Coleman EnsignDavid J. BowenJennifer L. TenorTodd A. CicheJames K. PetellJames Arthur StricklandGregory L. OrrRaymond FatigScott B. BintrimRichard H. Ffrench-Constant
C07K 14/24Y10S435/822A01N 63/50Y02A40/146
55
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Claims

Abstract

Proteins from the genus Xenorhabdus are toxic to insects upon oral exposure. These protein toxins can be applied to insect larvae food and plants for insect control.

Claims

exact text as granted — not AI-modified
We claim:  
     
         1 . A composition, comprising an effective amount of a Xenorhabdus protein toxin having functional activity against an insect, said protein toxin being derived from a protein having a native molecular size of at least 100 kDa.  
     
     
         2 . The composition of  claim 1 , wherein the Xenorhabdus toxin having functional activity against an insect is produced by a purified culture of  Xenorhabdus nematophilus, Xenorhabdus poinarii, Xenorhabdus bovienii, Xenorhabdus beddingii  or Xenorhabdus species.  
     
     
         3 . The composition of  claim 2 , wherein said purified culture of Xenorhabdus selected from the group consisting of S. carp, X. Wi, X. nem, X. NH3,  X. riobravis,  GL 133B, DEX1, DEX2, DEX3, DEX4, DEX5, DEX6, DEX7, DEX8, ILM037, ILM039, ILM070, ILM078, ILM079, ILM080, ILM081, ILM082, ILM083, ILM084, ILM102, ILM103, ILM104, ILM129, ILM133, ILM135, ILM138, ILM142, ILM143, GLX26, GLX40, GLX166, SEX20, SEX76, and SEX180.  
     
     
         4 . The composition of  claim 1 , wherein the toxin having functional activity against an insect is produced by a purified culture of Xenorhabdus strain designated S. carp, X. Wi, X. nem, X. NH3,  X. riobravis,  GL 133B, DEX1, DEX2, DEX3, DEX4, DEX5, DEX6, DEX7, DEX8, ILM037, ILM039, ILM070, ILM078, ILM079, ILM080, ILM081, ILM082, ILM083, ILM084, ILM102, ILM103, ILM104, ILM129, ILM133, ILM135, ILM138, ILM142, ILM143, GLX26, GLX40, GLX166, SEX20, SEX76, and SEX180.  
     
     
         5 . The composition of  claim 4 , wherein the toxin having functional activity against an insect is a mixture of one or more toxins produced from purified cultures of Xenorhabdus.  
     
     
         6 . The composition of  claim 3  wherein the toxin having functional activity against an insect, said toxin being a mixture of one or more toxins, is produced from said purified cultures of Xenorhabdus, said purified cultures being selected from the group consisting of S. carp, X. Wi, X. nem, X. NH3,  X. riobravis,  GL 133B, DEX1, DEX2, DEX3, DEX4, DEX5, DEX6, DEX7, DEX8, ILM037, ILM039, ILM070, ILM078, ILM079, ILM080, ILM081, ILM082, ILM083, ILM084, ILM102, ILM103, ILM104, ILM129, ILM133, ILM135, ILM138, ILM142, ILM143, GLX26, GLX40, GLX166, SEX20, SEX76, and SEX180.  
     
     
         7 . The composition of  claim 1 , wherein the insect is of the order Coleoptera, Lepidoptera, Diptera, or Acarina.  
     
     
         8 . The composition of  claim 7 , wherein the insect species from order Coleoptera are selected from the group consisting of Corn Rootworm, Boll Weevil, wireworms, pollen beetles, flea beetles, seed beetles and Colorado potato beetle.  
     
     
         9 . The composition of  claim 7 , wherein the insect species from order Lepidoptera are selected from the group consisting of Beet Armyworm, European Corn Borer, Tobacco Hornworm, Tobacco Budworm, cabbage looper, black cutworm, corn earworm, codling moth, clothes moth, Indian mealmoth, leaf rollers, cabbage worm, cotton bollworm, bagworm, Eastern tent caterpillar, sod webworm and fall armyworm.  
     
     
         10 . The composition of  claim 7 , wherein the insect species from the order Diptera are selected from the group consisting of pea midge, carrot fly, cabbage root fly, turnip root fly, onion fly, crane fly, house fly, and various mosquito species.  
     
     
         11 . The composition of  claim 7  wherein the insects species from the order Acarina are selected from the group consisting of two-spotted spider mites, strawberry spider mites, broad mites, citrus red mite, European red mite, pear rust mite and tomato russet mite.  
     
     
         12 . A substantially pure microorganism culture comprising of Xenorhabdus strain selected from the group consisting of S. carp, X. Wi, X. nem, X. NH3,  X. riobravis,  GL 133cn , ILM037, ILM039, ILM070, ILM078, ILM079, ILM080, ILM081, ILM082, ILM083, ILM084, ILM102, ILM103, ILM104, ILM129, ILM133, ILM135, ILM138, ILM142, ILM143, GLX26, GLX40, GLX166, SEX20, SEX76, and SEX180.  
     
     
         13 . A purified protein preparation comprising, a Xenorhabdus protein with at least one subunit having an approximate molecular weight between about 20 kDa to about 350 kDa; between about 130 kDa to about 350 kDa; about 80 kDa to about 130 kDa; about 40 kDa to about 80 kDa; or about 20 kDa to about 40 kDa.  
     
     
         14 . The purified protein preparation of  claim 13  comprising, a native Xenorhabdus protein with at least one subunit having a molecular weight of at least 100 kDa or greater.  
     
     
         15 . A purified protein preparation comprising a protein containing an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 and SEQ ID NO:5.  
     
     
         16 . A method of controlling an insect comprising, delivering to an insect an effective amount of a protein toxin having functional activity against an insect, wherein the protein is produced by a purified bacterial culture of the genus Xenorhabdus and has an native molecular weight of at least 100 kDa.  
     
     
         17 . The method of  claim 16 , wherein the Xenorhabdus toxin having functional activity against an insect is produced by a purified culture of  Xenorhabdus nematophilus, Xenorhabdus poinarii, Xenorhabdus bovienii, Xenorhabdus beddingii  or Xenorhabdus species.  
     
     
         18 . The method of  claim 17 , wherein said purified culture of Xenorhabdus selected from the group consisting of S. carp, X. Wi, X. nem, X. NH3,  X. riobravis,  GL 133B, DEX1, DEX2, DEX3, DEX4, DEX5, DEX6, DEX7, DEX8, ILM037, ILM039, ILM070, ILM078, ILM079, ILM080, ILM081, ILM082, ILM083, ILM084, ILM102, ILM103, ILM104, ILM129, ILM133, ILM135, ILM138, ILM142, ILM143, GLX26, GLX40, GLX166, SEX20, SEX76, and SEX180.  
     
     
         19 . The method of  claim 16 , wherein the toxin having functional activity against an insect is produced by a purified culture of Xenorhabdus strain designated S. carp, X. Wi, X. nem, X. NH3, X. riobravis, GL 133B, DEX1, DEX2, DEX3, DEX4, DEX5, DEX6, DEX7, DEX8, ILM037, ILM039, ILM070, ILM078, ILM079, ILM080, ILM081, ILM082, ILM083, ILM084, ILM102, ILM103, ILM104, ILM129, ILM133, ILM135, ILM138, ILM142, ILM143, GLX26, GLX40, GLX166, SEX20, SEX76, and SEX180.  
     
     
         20 . The method of  claim 16 , wherein the toxin having functional activity against an insect is a mixture of one or more toxins produced from purified cultures of Xenorhabdus.  
     
     
         21 . The method of  claim 16 , wherein the insect is of the order Coleoptera, Lepidoptera, Diptera, or Acarina.  
     
     
         22 . The method of  claim 21 , wherein the insect species from order Coleoptera are selected from the group consisting of Corn Rootworm, Boll Weevil, wireworms, pollen beetles, flea beetles, seed beetles and Colorado potato beetle.  
     
     
         23 . The method of  claim 21 , wherein the insect species from order Lepidoptera are selected from the group consisting of Beet Armyworm, European Corn Borer, Tobacco Hornworm, Tobacco Budworm, cabbage looper, black cutworm, corn earworm, codling moth, clothes moth, Indian mealmoth, leaf rollers, cabbage worm, cotton bollworm, bagworm, Eastern tent caterpillar, sod webworm and fall armyworm.  
     
     
         24 . The method of  claim 21 , wherein the insect species from the order Diptera are selected from the group consisting of pea midge, carrot fly, cabbage root fly, turnip root fly, onion fly, crane fly, house fly, and various mosquito species.  
     
     
         25 . The method of  claim 21 , wherein the insect species from the order Acarina are selected from the group consisting of two-spotted spider mites, strawberry spider mites, broad mites, citrus red mite, European red mite, pear rust mite and tomato russet mite.  
     
     
         26 . A method of altering the toxin level or toxin composition produced by Xenorhabdus strains comprising, modifying media composition.  
     
     
         27 . The method of  claim 26  wherein said media composition is modified by fermenting said Xenorhabdus in tryptic soy broth.  
     
     
         28 . The method of  claim 26  wherein said media composition is modified by increasing ionic strength of said media.

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