Human DNA mismatch repair proteins

Abstract

The present invention discloses three human DNA repair proteins and DNA (RNA) encoding such proteins and a prodeudre for producing such proteins by recombinant techniques. One of the human DNA repair proteins, hMLH1, has been mapped to chromosome 3 while hMLH2 has been mapped to chromosome 2 and hMLH3 has been mapped to chromosome 7. The polynucleotide sequences of the DNA repair proteins may be used for therapeutic and diagnostic treatments of a hereditary susceptibility to cancer.

What is claimed is:  
     
         1 . An isolated polynucleotide comprising a member selected from the group consisting of: 
 (a) a polynucleotide encoding a polypeptide having the deduced amino acid sequence of SEQ ID NO:2 or a fragment of said polypeptide;    (b) a polynucleotide encoding a polypeptide having the amino acid sequence encoded by the cDNA contained in ATCC Deposit No. 75649;    (c) a polynucleotide encoding a polypeptide having the deduced amino acid sequence of SEQ ID NO:4 or a fragment of said polypeptide;    (d) a polynucleotide encoding a polypeptide having the amino acid sequence encoded by the cDNA contained in ATCC Deposit No. 75651;    (e) a polynucleotide encoding a polypeptide having the deduced amino acid sequence of SEQ ID NO:6 or a fragment of said polypeptide; and    (f) a polynucleotide encoding a polypeptide having the amino acid sequence encoded by the cDNA contained in ATCC Deposit No. 75650.    
     
     
         2 . The polynucleotide of  claim 1  wherein the polynucleotide is DNA.  
     
     
         3 . The polynucleotide of  claim 1  wherein said polynucleotide encodes a polypeptide having the deduced amino acid sequence of SEQ ID NO:2.  
     
     
         4 . The polynucleotide of  claim 1  wherein said polynucleotide encodes a polypeptide having the deduced amino acid sequence of SEQ ID NO:4.  
     
     
         5 . The polynucleotide of  claim 1  wherein said polynucleotide encodes a polypeptide having the deduced amino acid sequence of SEQ ID NO:6.  
     
     
         6 . The polynucleotide of  claim 1  wherein said polynucleotide encodes a polypeptide encoded by the cDNA of ATCC Deposit No. 75649.  
     
     
         7 . The polynucleotide of  claim 1  wherein said polynucleotide encodes a polypeptide encoded by the cDNA of ATCC Deposit No. 75651.  
     
     
         8 . The polynucleotide of  claim 1  wherein said polynucleotide encodes a polypeptide encoded by the cDNA of ATCC Deposit No. 75650.  
     
     
         9 . A vector containing the polynucleotide of  claim 1 .  
     
     
         10 . A host cell genetically engineered with the vector of  claim 9 .  
     
     
         11 . A process for producing a polypeptide comprising expressing from the host cell of  claim 10  the polypeptide encoded by said DNA.  
     
     
         12 . A process for producing cells capable of expressing a polypeptide comprising genetically engineering cells with the vector of  claim 9 .  
     
     
         13 . A polypeptide comprising a member selected from the group consisting of: 
 (a) a polypeptide having the deduced amino acid sequence of SEQ ID NO:2 and fragments thereof;    (b) a polypeptide encoded by the cDNA of ATCC Deposit No. 75649 and fragments of said polypeptide;    (c) a polypeptide having the deduced amino acid sequence of SEQ ID NO:4 and fragments thereof;    (d) a polypeptide encoded by the cDNA of ATCC Deposit No. 75651 and fragments of said polypeptide;    (e) a polypeptide having the deduced amino acid sequence of SEQ ID NO:6 and fragments thereof; and    (f) a polypeptide encoded by the cDNA of ATCC Deposit No. 75650 and fragments of said polypeptide.    
     
     
         14 . An antibody that specifically binds the polypeptide of  claim 13.