Human potassium channel genes

Abstract

Methods for isolating K+Hnov genes are provided. The K+Hnov nucleic acid compositions find use in identifying homologous or related proteins and the DNA sequences encoding such proteins; in producing compositions that modulate the expression or function of the protein; and in studying associated physiological pathways. In addition, modulation of the gene activity in vivo is used for prophylactic and therapeutic purposes, such as identification of cell type based on expression, and the like.

What is claimed is:  
     
         1 . An isolated nucleic acid encoding a mammalian K+Hnov protein.  
     
     
         2 . An isolated nucleic acid according to  claim 1 , wherein said K+Hnov protein has the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 25, 27, 30, 81 or 83.  
     
     
         3 . An isolated nucleic acid according to  claim 1 , wherein said K+Hnov protein has an amino acid sequence that is substantially identical to the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 25, 27, 30, 81 or 83.  
     
     
         4 . An isolated nucleic acid according to  claim 1  wherein the nucleotide sequence of said nucleic acid is SEQ ID NO:l, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 22, 23, 24, 26, 28, 29, 80 or 82.  
     
     
         5 . An isolated nucleic acid that hybridizes under stringent conditions to a nucleic acid sequence of  claim 4 .  
     
     
         6 . An expression cassette comprising a transcriptional initiation region functional in an expression host, a nucleic acid having a sequence of the isolated nucleic acid according to  claim 1  under the transcriptional regulation of said transcriptional initiation region, and a transcriptional termination region functional in said expression host.  
     
     
         7 . A cell comprising an expression cassette according to  claim 6  as part of an extrachromosomal element or integrated into the genome of a host cell as a result of introduction of said expression cassette into said host cell, and the cellular progeny of said host cell.  
     
     
         8 . A method for producing mammalian K+Hnov protein, said method comprising: 
 growing a cell according to  claim 7 , whereby said mammalian K+Hnov protein is expressed; and    isolating said K+Hnov protein free of other proteins.    
     
     
         9 . A purified polypeptide composition comprising at least 50 weight % of the protein present as a K+Hnov protein or a fragment thereof.  
     
     
         10 . A monoclonal antibody binding specifically to a K+Hnov protein.  
     
     
         11 . A non-human transgenic animal model for K+Hnov gene function wherein said transgenic animal comprises an introduced alteration in a K+Hnov gene.  
     
     
         12 . The animal model of  claim 11 , wherein said animal is heterozygous for said introduced alteration.  
     
     
         13 . The animal model of  claim 12 , wherein said animal is homozygous for said introduced alteration.  
     
     
         14 . The animal model of  claim 12 , wherein said introduced alteration is a knockout of endogenous K+Hnov gene expression.