Dopamine receptors and genes

Abstract

A mammalian D 2 dopamine receptor gene has been cloned. Thus, DNA sequences encoding all or a part of the dopamine receptor are provided, as well as the corresponding polypeptide sequences and methods for producing the same both synthetically and via expression of a corresponding sequence from a host transformed with a suitable vector carrying the corresponding DNA sequence. The various structural information provided by this invention enables the preparation of labeled or unlabeled immunospecific species, particularly antibodies, as well as nucleic acid probes labeled in conventional fashion. Pharmaceutical compositions and methods of using various products of this invention are also provided.

What is claimed is:  
     
         1 . An antibody capable of specifically binding to a mammalian D2 dopamine receptor having an amino acid sequence identified as the amino acid sequence of FIGS.  7 A-C, FIGS.  18 A-H or FIGS.  18 A-H wherein amino acids 242-270 are deleted therefrom.  
     
     
         2 . An antibody according to  claim 1  that is a monoclonal antibody.  
     
     
         3 . An antigen-binding fragment of an antibody according to  claim 1 , wherein said fragment can be produced by chemical or enzymatic cleavage of said antibody.  
     
     
         4 . An antigen-binding fragment according to  claim 3 , wherein the fragment is an Fab fragment, an F(ab)′ fragment, an F(ab) 2  fragment or an Fv fragment.  
     
     
         5 . An antibody according to  claim 1  wherein the mammalian D2 dopamine receptor is a human D2 dopamine receptor.  
     
     
         6 . An antibody according to  claim 5  wherein the mammalian D2 dopamine receptor has an amino acid sequence identified as the amino acid sequence of FIGS.  18 A-H or FIGS.  18 A-H wherein amino acids 242-270 are deleted therefrom.  
     
     
         7 . An antibody according to  claim 1  wherein the mammalian D2 dopamine receptor is a rat D2 dopamine receptor.  
     
     
         8 . An antibody according to  claim 5  wherein the mammalian D2 dopamine receptor has an amino acid sequence identified as the amino acid sequence of FIGS.  7 A-C.  
     
     
         9 . An antibody according to  claim 1  wherein the antibody is detectably-labeled.  
     
     
         10 . An antibody according to  claim 1  where the antibody has immunological binding specificity for an epitope comprising amino acids 2-13, 182-192, 264-277, 289-298 or 404-414 in FIG. 1.